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Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-03-03 , DOI: 10.1039/c9lc01213c
Jinzhu Yu 1 , Ki Oh 1 , Sitapriya Moorthi 2 , Ling Li 1 , Helmut H Strey 3 , Michael Schuster 4 , Chiara Luberto 2 , Phenix-Lan Quan 1 , Eric Brouzes 5
Affiliation  

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

中文翻译:

基于酶的细胞计数法,一种敏感的单细胞细胞计数法,用于评估CML中的BCR-ABL1活性。

我们开发了一个简单,快速且具有成本效益的基于酶的细胞计数平台,以测量细胞内信号通路的活性。我们的单细胞微孔阵列平台使用酶信号放大并利用Michaelis-Menten动力学来定量蛋白质磷酸化。与传统的流式细胞仪相比,我们的方法可将分辨率提高两倍。
更新日期:2020-03-03
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