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Single cell migration profiling on a microenvironmentally tunable hydrogel microstructure device that enables stem cell potency evaluation.
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-03-03 , DOI: 10.1039/c9lc00988d
Enrique Ros 1 , Matías Encina 1 , Fabián González 1 , Rafael Contreras 2 , Patricia Luz-Crawford 2 , Maroun Khoury 3 , Juan Pablo Acevedo 3
Affiliation  

Cell migration is a key function in a myriad of physiological events and disease conditions. Efficient, quick and descriptive profiling of migration behaviour in response to different treatments or conditions is highly desirable in a series of applications, ranging from fundamental studies of the migration mechanism to drug discovery and cell therapy. This investigation applied the use of methacrylamide gelatin (GelMA) to microfabricate migration lanes based on GelMA hydrogel with encapsulated migration stimuli and structural stability under culture medium conditions, providing the possibility of tailoring the microenvironment during cell-based assays. The actual device provides 3D topography, cell localization and a few step protocol, allowing the quick evaluation and quantification of individual migrated distances of a cell sample by an ImageJ plugin for automated microscopy processing. The detailed profiling of migration behaviour given by the new device has demonstrated a broader assay sensitivity compared to other migration assays and higher versatility to study cell migration in different settings of applications. In this study, parametric information extracted from the migration profiling was successfully used to develop predictive models of immunosuppressive cell function that could be applied as a potency test for mesenchymal stem cells.

中文翻译:

在微环境可调水凝胶微结构设备上进行单细胞迁移分析,可进行干细胞效能评估。

细胞迁移是无数生理事件和疾病状况中的关键功能。从迁移机理的基础研究到药物发现和细胞疗法,在一系列应用中,非常需要对迁移方法进行有效,快速和描述性描述的迁移行为。这项研究将甲基丙烯酰胺明胶(GelMA)应用于基于GelMA水凝胶的微制造迁移通道,并在培养基条件下具有封装的迁移刺激和结构稳定性,从而提供了在基于细胞的测定过程中调整微环境的可能性。实际的设备提供3D地形,单元定位和几步协议,允许通过ImageJ插件快速评估和量化细胞样品的单个迁移距离,以进行自动显微镜处理。与其他迁移测定法相比,新设备给出的迁移行为的详细分析证明了测定法灵敏度更高,并且在不同应用场合下研究细胞迁移的通用性更高。在这项研究中,从迁移分析中提取的参数信息已成功用于开发免疫抑制细胞功能的预测模型,该模型可以用作间充质干细胞的效能测试。与其他迁移测定法相比,新设备给出的迁移行为的详细分析证明了测定法灵敏度更高,并且在不同应用场合下研究细胞迁移的通用性更高。在这项研究中,从迁移分析中提取的参数信息已成功用于建立免疫抑制细胞功能的预测模型,该模型可以用作间充质干细胞的效能测试。与其他迁移测定法相比,新设备给出的迁移行为的详细分析证明了测定法灵敏度更高,并且在不同应用场合下研究细胞迁移的通用性更高。在这项研究中,从迁移分析中提取的参数信息已成功用于建立免疫抑制细胞功能的预测模型,该模型可以用作间充质干细胞的效能测试。
更新日期:2020-03-03
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