Rapid and specific duplex detection of methicillin-resistant Staphylococcus aureus genes by surface-enhanced Raman spectroscopy.,Analyst

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Rapid and specific duplex detection of methicillin-resistant Staphylococcus aureus genes by surface-enhanced Raman spectroscopy.
Analyst ( IF 3.6 ) Pub Date : 2020/01/30 , DOI: 10.1039/c9an01959f
Phani R Potluri ₁ , Vinoth Kumar Rajendran , Anwar Sunna , Yuling Wang

Affiliation

Methicillin-resistant Staphylococcus aureus (MRSA) is considered to be one of the important hospital-acquired pathogens. MRSA is also commonly associated with hospital-acquired infections and mortality. Quantitative and precise detection of MRSA is essential for rapid diagnosis and subsequent effective disease management strategies. We herein developed a highly specific method for rapid MRSA detection that combines surface-enhanced Raman spectroscopy (SERS) nanotags and polymerase chain reaction (PCR). SERS provided the sensitivity and spectral multiplexing capability while PCR provided the specificity required for the assay. The method was tested by the simultaneous detection of two MRSA specific genes (mecA and femA) amplified from genomic DNA isolated from clinical specimens. Magnetic isolation and rapid duplex detection were performed to obtain a detectable signal down to 104 input copies within 80 min. This demonstrated the potential of the SERS-PCR based approach for the accurate identification of MRSA at an early-diagnosis stage. This study also provides an alternative approach to the existing methods for detecting clinical targets without compromising sensitivity and selectivity, and with minimal handling steps. We thus believe that this approach will find a broad application in clinical applications.

中文翻译：

通过表面增强拉曼光谱法快速，特异地双重检测耐甲氧西林的金黄色葡萄球菌基因。

耐甲氧西林金黄色葡萄球菌（MRSA）是医院获得的重要病原体之一。MRSA通常还与医院获得性感染和死亡率有关。MRSA的定量和精确检测对于快速诊断和后续有效的疾病管理策略至关重要。我们在此开发了一种高度特异性的快速MRSA检测方法，该方法结合了表面增强拉曼光谱（SERS）纳米标签和聚合酶链反应（PCR）。SERS提供了灵敏度和光谱多路复用功能，而PCR提供了测定所需的特异性。通过同时检测从临床标本中分离的基因组DNA扩增的两个MRSA特异性基因（mecA和femA）来测试该方法。进行了磁隔离和快速双工检测，以在80分钟内获得多达104个输入副本的可检测信号。这证明了基于SERS-PCR的方法在早期诊断阶段准确鉴定MRSA的潜力。这项研究还为检测临床目标的现有方法提供了另一种方法，而又不影响灵敏度和选择性，并且只需最少的处理步骤。因此，我们认为这种方法将在临床应用中找到广泛的应用。这项研究还为检测临床目标的现有方法提供了另一种方法，而又不影响灵敏度和选择性，并且只需最少的处理步骤。因此，我们认为这种方法将在临床应用中找到广泛的应用。这项研究还为检测临床目标的现有方法提供了另一种方法，而又不影响灵敏度和选择性，并且只需最少的处理步骤。因此，我们认为这种方法将在临床应用中找到广泛的应用。

更新日期：2020-03-31

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