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Fluorescence resonance energy transfer (FRET)-based nanoarchitecture for monitoring deubiquitinating enzyme activity.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-02-18 , DOI: 10.1039/c9cc09808a Yan-Yan Liang 1 , Jie Zhang , Hui Cui , Zhen-Shu Shao , Chen Cheng , Yue-Bo Wang , Huai-Song Wang
Chemical Communications ( IF 4.3 ) Pub Date : 2020-02-18 , DOI: 10.1039/c9cc09808a Yan-Yan Liang 1 , Jie Zhang , Hui Cui , Zhen-Shu Shao , Chen Cheng , Yue-Bo Wang , Huai-Song Wang
Affiliation
A novel nanoarchitecture (MSN-Tb-UbR) was prepared by modifying rhodamine B-labelled Ubs (Ub-Rs) on the surface of mesoporous silica nanoparticles (MSNs) loaded with Tb3+-complexes. The MSN-Tb-UbR exhibits ratiometric sensing ability for DUB (UCH-L1) with good sensitivity and selectivity.
中文翻译:
基于荧光共振能量转移(FRET)的纳米体系结构,用于监测去泛素化酶的活性。
通过修饰负载了Tb3 +复合物的中孔二氧化硅纳米粒子(MSN)表面上的若丹明B标记的Ub(Ub-Rs),制备了一种新型的纳米结构(MSN-Tb-UbR)。MSN-Tb-UbR具有对DUB(UCH-L1)的比例感测能力,具有良好的灵敏度和选择性。
更新日期:2020-03-12
中文翻译:
基于荧光共振能量转移(FRET)的纳米体系结构,用于监测去泛素化酶的活性。
通过修饰负载了Tb3 +复合物的中孔二氧化硅纳米粒子(MSN)表面上的若丹明B标记的Ub(Ub-Rs),制备了一种新型的纳米结构(MSN-Tb-UbR)。MSN-Tb-UbR具有对DUB(UCH-L1)的比例感测能力,具有良好的灵敏度和选择性。