Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1 mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant.,Photochemical & Photobiological Sciences

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Ultraviolet-B radiation exposure lowers the antioxidant capacity in the Arabidopsis thaliana pdx1.3-1 mutant and leads to glucosinolate biosynthesis alteration in both wild type and mutant.
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020-01-21 , DOI: 10.1039/c9pp00342h
Susanne Neugart ₁ , Éva Hideg , Gyula Czégény , Monika Schreiner , Åke Strid

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Pyridoxine (vitamin B6) and its vitamers are used by living organisms both as enzymatic cofactors and as antioxidants. We used Arabidopsis pyridoxine biosynthesis mutant pdx1.3-1 to study the involvement of the PLP-synthase main polypeptide PDX1 in plant responses to ultraviolet radiation of two different qualities, one containing primarily UV-A (315-400 nm) and the other containing both UV-A and UV-B (280-315 nm). The antioxidant capacity and the flavonoid and glucosinolate (GS) profiles were examined. As an indicator of stress, Fv/Fm of photosystem II reaction centers was used. In pdx1.3-1, UV-A + B exposure led to a significant 5% decrease in Fv/Fm on the last day (day 15), indicating mild stress at this time point. The antioxidant capacity of Col-0 wildtype increased significantly (50-73%) after 1 and 3 days of UV-A + B. Instead, in pdx1.3-1, the antioxidant capacity significantly decreased by 44-52% over the same time period, proving the importance of a full complement of functional PDX1 genes for the detoxification of reactive oxygen species. There were no significant changes in the flavonoid glycoside profile under any light condition. However, the GS profile was significantly altered, both with respect to Arabidopsis accession and exposure to UV. The difference in flavonoid and GS profiles reflects that the GS biosynthesis pathway contains at least one pyridoxine-dependent enzyme, whereas no such enzyme is used in flavonoid biosynthesis. Also, there was strong correlation between the antioxidant capacity and the content of some GS compounds. Our results show that vitamin B6 vitamers, functioning both as antioxidants and co-factors, are of importance for the physiological fitness of plants.

中文翻译：

紫外线B辐射降低了拟南芥pdx1.3-1突变体的抗氧化能力，并导致野生型和突变体中芥子油苷的生物合成改变。

吡rid醇（维生素B6）及其维他命被活生物体用作酶促辅助因子和抗氧化剂。我们使用拟南芥吡ido醇生物合成突变体pdx1.3-1研究了PLP合酶主要多肽PDX1参与植物对两种不同质量的紫外线辐射的响应，其中一种主要包含UV-A（315-400 nm），而另一种则包含UV-A和UV-B（280-315 nm）。检查了抗氧化能力以及类黄酮和芥子油苷（GS）谱。使用光系统II反应中心的Fv / Fm作为压力指标。在pdx1.3-1中，UV-A + B暴露导致最后一天（第15天）的Fv / Fm显着降低5％，表明此时该压力较小。在UV-A + B放置1天和3天后，Col-0野生型的抗氧化能力显着提高（50-73％）。相反，在pdx1.3-1中，同时，抗氧化能力显着下降了44-52％，证明了功能性PDX1基因的完整互补对活性氧的解毒具有重要意义。在任何光照条件下，类黄酮糖苷谱均无明显变化。但是，无论是拟南芥的加入还是暴露于紫外线，GS的分布都发生了显着变化。类黄酮和GS谱的差异反映了GS生物合成途径包含至少一种吡dependent醇依赖性酶，而黄酮类生物合成中没有使用这种酶。此外，抗氧化能力与某些GS化合物的含量之间也存在很强的相关性。我们的结果表明，维生素B6维生素既可以作为抗氧化剂，也可以作为辅助因子，

更新日期：2020-02-19

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