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Smart wound dressing for infection monitoring and NIR-triggered antibacterial treatment.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-01-23 , DOI: 10.1039/c9bm02060h Bianbian Qiao 1 , Qian Pang 1 , Peiqi Yuan 1 , Yilun Luo 1 , Lie Ma 1
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-01-23 , DOI: 10.1039/c9bm02060h Bianbian Qiao 1 , Qian Pang 1 , Peiqi Yuan 1 , Yilun Luo 1 , Lie Ma 1
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Wound infection is a major challenge in the clinic that greatly hinders the wound healing process. It is highly important to develop smart wound dressings that can sense bacterial infection at early stages and provide on-demand treatment. In this work, a smart hydrogel-based wound dressing capable of monitoring bacterial infection via a pH-responsive fluorescence resonance energy transfer (FRET) transition of Cyanine3 (Cy3) and Cyanine5 (Cy5) in a bacterial environment and providing on-demand treatment of infection via near infrared (NIR) light-triggered antibiotic release was developed. The smart hydrogel was prepared by physical crosslinking of polyvinyl alcohol (PVA) and an ultraviolet (UV)-cleavable polyprodrug (GS-Linker-MPEG), in which Cy3 and Cy5-modified silica nanoparticles (SNP-Cy3/Cy5) were loaded and acted as a pH-responsive fluorescent probe to detect bacterial infection based on the FRET effect between Cy3 and Cy5. Also, up-conversion nanoparticles (UCNP) were loaded into the hydrogels to cleave the UV-responsive GS-Linker-MPEG and achieve NIR-responsive release of GS in the bacterial environment. The in vitro studies proved that the smart hydrogels present good water absorption ability and excellent mechanical properties as well as good biocompatibility, which are necessary for their application in wound dressings. Moreover, the hydrogels showed obvious FRET transitions in both acidic buffer and bacteria solution. Upon irradiating the hydrogels with NIR light, UCNP were able to convert NIR light to UV light to trigger the release of GS from the hydrogels for antibacterial treatment. This research is expected to provide a new strategy for self-reporting and effective treatment of wound infection.
中文翻译：
智能伤口敷料，用于感染监测和近红外触发的抗菌治疗。
伤口感染是临床上的主要挑战，极大地阻碍了伤口的愈合过程。开发能够在早期阶段感觉到细菌感染并按需提供治疗的智能伤口敷料非常重要。在这项工作中，基于水凝胶的智能伤口敷料能够通过在细菌环境中通过Cyanine3（Cy3）和Cyanine5（Cy5）的pH响应荧光共振能量转移（FRET）转变来监测细菌感染，并按需提供治疗开发了通过近红外（NIR）光触发的抗生素释放引起的感染。智能水凝胶是通过聚乙烯醇（PVA）和可紫外线（UV）裂解的聚前药（GS-Linker-MPEG）物理交联制备的，其中装载Cy3和Cy5修饰的二氧化硅纳米颗粒（SNP-Cy3 / Cy5），并充当pH响应荧光探针，根据Cy3和Cy5之间的FRET效应检测细菌感染。同样，将上转换纳米颗粒（UCNP）装入水凝胶中，以裂解紫外线响应的GS-Linker-MPEG，并在细菌环境中实现GS的近红外响应释放。体外研究证明，智能水凝胶具有良好的吸水能力，优异的机械性能以及良好的生物相容性，这对于将其应用于伤口敷料是必不可少的。此外，水凝胶在酸性缓冲液和细菌溶液中均表现出明显的FRET转变。用近红外光照射水凝胶后，UCNP能够将NIR光转换为UV光，从而触发GS从水凝胶中释放出来进行抗菌处理。该研究有望为自我报告和有效治疗伤口感染提供新的策略。
更新日期：2020-03-19
中文翻译：
智能伤口敷料，用于感染监测和近红外触发的抗菌治疗。
伤口感染是临床上的主要挑战，极大地阻碍了伤口的愈合过程。开发能够在早期阶段感觉到细菌感染并按需提供治疗的智能伤口敷料非常重要。在这项工作中，基于水凝胶的智能伤口敷料能够通过在细菌环境中通过Cyanine3（Cy3）和Cyanine5（Cy5）的pH响应荧光共振能量转移（FRET）转变来监测细菌感染，并按需提供治疗开发了通过近红外（NIR）光触发的抗生素释放引起的感染。智能水凝胶是通过聚乙烯醇（PVA）和可紫外线（UV）裂解的聚前药（GS-Linker-MPEG）物理交联制备的，其中装载Cy3和Cy5修饰的二氧化硅纳米颗粒（SNP-Cy3 / Cy5），并充当pH响应荧光探针，根据Cy3和Cy5之间的FRET效应检测细菌感染。同样，将上转换纳米颗粒（UCNP）装入水凝胶中，以裂解紫外线响应的GS-Linker-MPEG，并在细菌环境中实现GS的近红外响应释放。体外研究证明，智能水凝胶具有良好的吸水能力，优异的机械性能以及良好的生物相容性，这对于将其应用于伤口敷料是必不可少的。此外，水凝胶在酸性缓冲液和细菌溶液中均表现出明显的FRET转变。用近红外光照射水凝胶后，UCNP能够将NIR光转换为UV光，从而触发GS从水凝胶中释放出来进行抗菌处理。该研究有望为自我报告和有效治疗伤口感染提供新的策略。
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