当前位置: X-MOL 学术Clin. Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
HDL-associated apoCIII plays an independent role in predicting postprandial hypertriglyceridemia.
Clinical Biochemistry ( IF 2.5 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.clinbiochem.2020.02.004
Tianhua Zhang 1 , Xiaoyu Tang 1 , Ling Mao 1 , Jin Chen 1 , Jie Kuang 1 , Xin Guo 1 , Danyan Xu 1 , Daoquan Peng 1 , Bilian Yu 1
Affiliation  

BACKGROUND The mechanism for an abnormal pattern of triglyceride (TG) metabolism in response to a meal still needs further investigation. Extensive pieces of evidence have shown that apolipoprotein CIII (apoCIII) is a critical modulator of plasma TG metabolism mostly by inhibiting the hydrolysis of TG. Little is known about the role of apoCIII contained in high density lipoprotein (HDL) in plasma TG metabolism after a meal. METHODS Fasting and 4-hour postprandial peripheral venous blood were collected in 91 subjects selected from our hospital. Serum lipid parameters, apoCIII levels and HDL subcomponents were tested by standard laboratory procedures, ELISA, and nuclear magnetic resonance (NMR), respectively. The t-test, and Non-parametric tests were performed to examine differences between groups, Pearson's correlation and multiple regression analysis were used to assess the correlations between apoCIII (HDL-associated or nonHDL-associated) and postprandial TG. RESULTS There was a significant increase in TG after a meal compared to fasting status [155.40(96.70-251.07) mg/dl.vs.118.53(83.38-173.29)mg/dl, p < 0.001]. However, the total apoCIII levels were unchanged before (11.56(7.89-16.22) mg/dl) and after a meal (11.66(7.75-16.02)mg/dl, p = 0.124), while a significant increase in HDL-associated apoCIII (HDL-apoCIII) was observed from fasting (5.25(3.92-7.83)mg/dl) to post-meal (6.46(4.57-8.76)mg/dl, p = 0.001). Unlike nonHDL-apoCIII, HDL-apoCIII was positively correlated with both fasting and postprandial plasma TG in subjects with baseline plasma TG > 118.53 mg/dl (R = 0.503, p < 0.001 for fasting, R = 0.584, p < 0.001 for postprandial). Besides, in the subjects who had an abnormal TG response to a meal, which was defined as postprandial plasma TG increase of>30% compared to baseline TG levels, postprandial HDL-apoCIII was also increased significantly [5.37(3.52-7.02)mg/dl.vs.6.64(4.61-8.86)mg/dl, p = 0.001]. The enrichment of apoCIII in HDL led to changes of TG, cholesterol, free cholesterol, phospholipid and apoAII contents in HDL particles defined by NMR. CONCLUSION Enrichment of apoCIII in HDL particles potentially plays an independent role in postprandial hypertriglyceridemia.

中文翻译:

HDL相关的apoCIII在预测餐后高甘油三酯血症中起独立作用。

背景技术餐后甘油三酸酯(TG)代谢异常模式的机制仍需进一步研究。大量证据表明,载脂蛋白CIII(apoCIII)是血浆TG代谢的关键调节剂,主要是通过抑制TG的水解来实现的。餐后血浆TG代谢中高密度脂蛋白(HDL)中所含apoCIII的作用鲜为人知。方法收集我院91例患者的空腹和餐后4小时外周血。血清脂质参数,apoCIII水平和HDL子成分分别通过标准实验室程序,ELISA和核磁共振(NMR)进行测试。进行t检验和非参数检验以检验组之间的差异,Pearson' 的相关性和多元回归分析用于评估apoCIII(与HDL相关或与非HDL相关)与餐后TG之间的相关性。结果与禁食相比,餐后TG显着增加[155.40(96.70-251.07)mg / dl.vs.118.53(83.38-173.29)mg / dl,p <0.001]。然而,餐前(11.56(7.89-16.22)mg / dl)和餐后(11.66(7.75-16.02)mg / dl,p = 0.124)的总apoCIII水平没有变化,而与HDL相关的apoCIII显着增加(从禁食(5.25(3.92-7.83)mg / dl)到餐后(6.46(4.57-8.76)mg / dl,p = 0.001)观察到HDL-apoCIII)。与非HDL-apoCIII不同,在基线血浆TG> 118.53 mg / dl的受试者中,HDL-apoCIII与空腹和餐后血浆TG呈正相关(R = 0.503,空腹p <0.001,R = 0.584,p <0。餐后001)。此外,对于餐后TG反应异常的受试者(定义为餐后血浆TG与基线TG水平相比增加> 30%),餐后HDL-apoCIII也显着增加[5.37(3.52-7.02)mg / dl.vs.6.64(4.61-8.86)mg / dl,p = 0.001]。HDL中apoCIII的富集导致NMR定义的HDL颗粒中TG,胆固醇,游离胆固醇,磷脂和apoAII含量发生变化。结论HDL颗粒中apoCIII的富集可能在餐后高甘油三酯血症中起独立作用。001]。HDL中apoCIII的富集导致NMR定义的HDL颗粒中TG,胆固醇,游离胆固醇,磷脂和apoAII含量发生变化。结论HDL颗粒中apoCIII的富集可能在餐后高甘油三酯血症中起独立作用。001]。HDL中apoCIII的富集导致NMR定义的HDL颗粒中TG,胆固醇,游离胆固醇,磷脂和apoAII含量发生变化。结论HDL颗粒中apoCIII的富集可能在餐后高甘油三酯血症中起独立作用。
更新日期:2020-02-08
down
wechat
bug