PTMs such as phosphorylations are usually involved in signal transduction pathways. To investigate the temporal dynamics of phosphoproteome changes upon viral infection, a model system of IMR‐90 cells infected with human adenovirus type 2 (Ad2) is used in a time‐course quantitative analysis combining titanium dioxide (TiO₂) particle enrichment and SILAC‐MS. Quantitative data from 1552 phosphorylated sites clustered the highly altered phosphorylated sites to the signaling by rho family GTPases, the actin cytoskeleton signaling, and the cAMP‐dependent protein kinase A signaling pathways. Their activation is especially pronounced at early time post‐infection. Changes of several phosphorylated sites involved in the glycolysis pathway, related to the activation of the Warburg effect, point at virus‐induced energy production. For Ad2 proteins, 32 novel phosphorylation sites are identified and as many as 52 phosphorylated sites on 17 different Ad2 proteins are quantified, most of them at late time post‐infection. Kinase predictions highlighted activation of PKA, CDK1/2, MAPK, and CKII. Overlaps of kinase motif sequences for viral and human proteins are observed, stressing the importance of phosphorylation during Ad2 infection.

中文翻译：

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腺病毒2型感染过程中反应的磷酸化时间过程研究。

PTM（例如磷酸化）通常参与信号转导途径。为了研究病毒感染后磷酸化蛋白质组变化的时间动态，将感染了人类2型腺病毒（Ad2）的IMR-90细胞模型系统用于结合二氧化钛（TiO ₂）颗粒富集和SILAC-MS。来自1552个磷酸化位点的定量数据将高度改变的磷酸化位点聚集到rho家族GTPases，肌动蛋白细胞骨架信号转导和cAMP依赖性蛋白激酶A信号通路的信号转导中。它们的激活在感染后的早期特别明显。糖酵解途径中涉及的几个磷酸化位点的变化，与Warburg效应的激活有关，指向病毒诱导的能量产生。对于Ad2蛋白，已鉴定出32个新的磷酸化位点，并且对17种不同的Ad2蛋白上多达52个磷酸化位点进行了定量，其中大多数在感染后较晚。激酶预测强调了PKA，CDK1 / 2，MAPK和CKII的激活。观察到病毒和人类蛋白质的激酶基序序列重叠，