Postoperative depression is a serious problem with under-optimized treatment. Our previous result demonstrated that single dose of propofol exposure in mice could cause depressive-like behavior. In the present study, we explored the possible preventive strategy that could ameliorate the depressive-like behavior in mice. Irisin was used for co-treatment with propofol in mice to test whether irisin could prevent the depressive-like behavior in mice. We found irisin could significantly reduce the immobility time in tail suspension and forced swimming tests in mice with propofol. We also found irisin was able to protect neurons against high concentration propofol treatment induced cell death in cultures. Meanwhile, irisin could significantly inhibit the increase of cytokines in astrocyte cultures exposed to propofol. These in vitro results suggested that interaction between astrocyte and neuron may be involved in the action mechanism that irisin prevented the mice from developing of depressive-like behavior. Last, we revealed that propofol caused increased surface expression of epidermal growth factor receptor (EGFR) but irisin could reduce the expression level of EGFR on cell surface.

中文翻译：

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Irisin通过减少小鼠中表皮生长因子受体的表面表达来改善术后抑郁样行为。

术后抑郁症是治疗不足的严重问题。我们以前的结果表明，在小鼠中单剂量的异丙酚暴露可能引起抑郁样行为。在本研究中，我们探讨了可能改善小鼠抑郁样行为的预防策略。Irisin被用于与丙泊酚在小鼠中的共同治疗，以测试鸢尾素是否可以预防小鼠的抑郁样行为。我们发现鸢尾素可以显着减少丙泊酚小鼠的尾部悬吊和强迫游泳试验中的固定时间。我们还发现鸢尾素能够保护神经元免受高浓度丙泊酚治疗诱导的细胞死亡。同时，鸢尾素可以显着抑制暴露于异丙酚的星形胶质细胞培养物中细胞因子的增加。这些体外结果表明，星形胶质细胞和神经元之间的相互作用可能参与了虹膜蛋白阻止小鼠发展成抑郁样行为的作用机制。最后，我们揭示了丙泊酚引起表皮生长因子受体（EGFR）的表面表达增加，但是鸢尾素可以降低细胞表面EGFR的表达水平。