This study investigated the role of the feeding regime on cellular (lysosomal membrane impairment), oxidative (superoxides and nitric oxides generation, as well as lipid peroxidation) and genotoxic (nuclear abnormalities) biomarkers measured in hemocytes of mussels Mytilus galloprovincialis treated with diclofenac (DCF). Specifically, unfed mussels, or mussels fed ad libitum with algal species Tisochrysis lutea or Tetraselmis suecica (Tiso/DCF- and Tetra/DCF- treated mussels, respectively) were exposed to DCF (20 μgL-1) for 4 days. The results showed that biomarkers' responsiveness against DCF, were more pronounced in unfed and Tetra/DCF-, rather than Tiso/DCF- treated mussel hemocytes, thus revealing food deprivation, changes in mussel feeding/filtration rate and digestion processes, as potent factors of mussels' immune efficiency and response against DCF. Those findings could provide valuable data for the optimization of mussels' feeding regime during laboratory studies, in order to assess reliably the effects of emerging contaminants on non-target sentinel organisms, such as mussels.

中文翻译：

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喂食方式可调节用双氯芬酸处理过的贻贝中的生物标记物反应性。

这项研究调查了饲喂方案对用双氯芬酸（DCF）处理的贻贝Mytilus galloprovincialis血细胞中测量的细胞（溶酶体膜损伤），氧化（超氧化物和一氧化氮生成以及脂质过氧化）和遗传毒性（核异常）生物标志物的作用。 ）。具体来说，将未喂食的贻贝或随意喂食藻种黄褐线虫或Tetraselmis suecica的贻贝（分别用Tiso / DCF和Tetra / DCF处理的贻贝）暴露于DCF（20μgL-1）4天。结果表明，生物标记物对DCF的反应在未进食和Tetra / DCF-处理中比在Tiso / DCF处理过的贻贝血细胞中更为明显，因此揭示了食物缺乏，贻贝进食/过滤速率和消化过程的变化是有效的因素贻贝 免疫效率和对DCF的反应。这些发现可以为优化贻贝在实验室研究期间的进食方式提供有价值的数据，以便可靠地评估新兴污染物对诸如贻贝之类的非目标定点生物的影响。