The present pilot study aimed at evaluating air sampling as a novel method for monitoring Campylobacter in poultry farms. We compared the bacteriological isolation of Campylobacter from boot swabs and air filter samples using ISO 10272-1:2017. A secondary aim was to evaluate the use of molecular methods, i.e. real time PCR, on the same sample set. Samples from 44 flocks from five European countries were collected, and included air samples, in parallel with boot swabs. Campylobacter spp. was isolated from seven of 44 boot swabs from three of five partners using the enrichment method. Two of these positive boot swab samples had corresponding positive air samples. Using enrichment, one positive air sample was negative in the corresponding boot swabs, but Campylobacter spp. was isolated from direct plating of the boot swab sample. One partner isolated Campylobacter spp. from six of 10 boot swabs using direct plating. Overall, 33 air filter samples were screened directly with PCR, returning 14 positive results. In conclusion, there was a lack of correspondence between results from analysis of boot swabs and air filters using ISO 10272-1:2017. In contrast, the combination of air filters and direct real-time PCR might be a way forward. Despite the use of the detailed ISO protocols, there were still sections that could be interpreted differently among laboratories. Air sampling may turn into a multi-purpose and low-cost sampling method that may be integrated into self-monitoring programs.

中文翻译：

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鸡肉中的弯曲杆菌-国际上多中心评估空气采样和检测方法的关键参数。

当前的初步研究旨在评估空气采样，作为监测家禽养殖场弯曲杆菌的一种新方法。我们使用ISO 10272-1：2017比较了从拭子和空气过滤器样品中弯曲杆菌的细菌学分离。第二个目的是评估在同一样本集上分子方法（即实时PCR）的使用。收集了来自五个欧洲国家的44个鸡群的样品，其中包括空气样品以及棉签。弯曲杆菌属。使用富集方法从五个伙伴中的三个伙伴的44个靴子拭子中分离出七个。这些阳性棉签样本中有两个具有相应的阳性空气样本。使用富集，相应的引导拭子中有一个阳性空气样本为阴性，而弯曲杆菌属则为阴性。从引导拭子样品的直接铺板中分离得到。一位伴侣分离了弯曲杆菌属。使用直接电镀从10个棉签中的6个中取出。总体而言，直接用PCR筛选了33个空气滤清器样品，返回14个阳性结果。总之，使用ISO 10272-1：2017对行李箱拭子和空气过滤器的分析结果之间缺乏对应关系。相反，将空气过滤器和直接实时PCR结合使用可能是一种前进的道路。尽管使用了详细的ISO协议，但仍有部分实验室之间的解释可能有所不同。空气采样可能会变成一种多用途且低成本的采样方法，可以集成到自我监测程序中。使用ISO 10272-1：2017对行李箱拭子和空气过滤器的分析结果之间缺乏对应关系。相反，将空气过滤器和直接实时PCR结合使用可能是一种前进的道路。尽管使用了详细的ISO协议，但仍有部分实验室之间的解释可能有所不同。空气采样可能会变成一种多用途且低成本的采样方法，可以集成到自我监测程序中。使用ISO 10272-1：2017对行李箱拭子和空气过滤器的分析结果之间缺乏对应关系。相反，将空气过滤器和直接实时PCR结合使用可能是一种前进的道路。尽管使用了详细的ISO协议，但仍有部分实验室之间的解释可能有所不同。空气采样可能会变成一种多用途且低成本的采样方法，可以集成到自我监测程序中。