当前位置：
X-MOL 学术
›
Exp. Gerontol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
The anti-aging effect of Scutellaria baicalensis Georgi flowers extract by regulating the glutamine-glutamate metabolic pathway in d-galactose induced aging rats.
Experimental Gerontology ( IF 3.3 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.exger.2020.110843 Jia Song 1 , Yu-Zhi Zhou 2 , Yi-Yuan Pang 2 , Li Gao 2 , Guan-Hua Du 3 , Xue-Mei Qin 2
Experimental Gerontology ( IF 3.3 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.exger.2020.110843 Jia Song 1 , Yu-Zhi Zhou 2 , Yi-Yuan Pang 2 , Li Gao 2 , Guan-Hua Du 3 , Xue-Mei Qin 2
Affiliation
|
Scutellaria baicalensis Georgi flowers is rich in flavonoids resources but not effectively exploited. This study aimed to investigate the anti-aging effects and potential mechanism of Scutellaria baicalensis Georgi flowers extract (SFE). The chemical components of the SFE were analyzed by UPLC-MS and the anti-aging effects of SFE were investigated in d-galactose (d-gal) induced aging rats by behavior examination and biochemical indexes, and the potential anti-aging mechanism of SFE were explored by 1H NMR-based liver metabolomics. Chemical composition research showed that 19 flavonoids were identified in SFE, and pharmacological research showed that SFE could significantly ameliorate spatial learning and memory ability. SFE could significantly regulate malondialdehyde (MDA), superoxide dismutase (SOD) and advanced glycation end products (AGEs). It also ameliorated the pathological abnormalities in liver. Additionally, anti-aging mechanism of SFE showed that total of 10 potential biomarkers were found by metabolomics techniques, which involved in 6 metabolic pathways. Among them, SFE could significantly increased the levels of d-glutamine and d-glutamate. Furthermore, the levels of glutamine and glutamate, and the levels of the key amino acids, enzymes and final product in the synthesis process of glutathione (GSH) were quantitatively determined in the liver by commercial kits and enzyme-linked immunosorbent assay. These results indicated that regulation of the glutamine-glutamate metabolic pathway is involved in the anti-aging effect of SFE in d-gal induced aging rats.
中文翻译：
黄芩花提取物通过调节谷氨酰胺-谷氨酸代谢途径对D-半乳糖诱导衰老大鼠的抗衰老作用。
黄芩花含有丰富的黄酮类资源但尚未得到有效开发利用。本研究旨在探讨黄芩花提取物（SFE）的抗衰老作用及其潜在机制。采用UPLC-MS分析SFE的化学成分，并通过行为学检查和生化指标研究SFE对d-半乳糖（d-gal）致衰老大鼠的抗衰老作用，探讨SFE潜在的抗衰老机制。通过基于 1H NMR 的肝脏代谢组学进行了探索。化学成分研究表明，SFE 中鉴定出 19 种黄酮类化合物，药理学研究表明，SFE 可以显着改善空间学习和记忆能力。 SFE 可以显着调节丙二醛 (MDA)、超氧化物歧化酶 (SOD) 和晚期糖基化终末产物 (AGE)。它还改善了肝脏的病理异常。此外，SFE的抗衰老机制表明，通过代谢组学技术共发现了10个潜在的生物标志物，涉及6条代谢途径。其中，SFE能显着提高D-谷氨酰胺和D-谷氨酸的水平。此外，通过商业试剂盒和酶联免疫吸附测定法定量测定肝脏中谷氨酰胺和谷氨酸的水平，以及谷胱甘肽（GSH）合成过程中的关键氨基酸、酶和最终产物的水平。这些结果表明，谷氨酰胺-谷氨酸代谢途径的调节参与了SFE对d-gal诱导的衰老大鼠的抗衰老作用。
更新日期：2020-02-10
中文翻译：
黄芩花提取物通过调节谷氨酰胺-谷氨酸代谢途径对D-半乳糖诱导衰老大鼠的抗衰老作用。
黄芩花含有丰富的黄酮类资源但尚未得到有效开发利用。本研究旨在探讨黄芩花提取物（SFE）的抗衰老作用及其潜在机制。采用UPLC-MS分析SFE的化学成分，并通过行为学检查和生化指标研究SFE对d-半乳糖（d-gal）致衰老大鼠的抗衰老作用，探讨SFE潜在的抗衰老机制。通过基于 1H NMR 的肝脏代谢组学进行了探索。化学成分研究表明，SFE 中鉴定出 19 种黄酮类化合物，药理学研究表明，SFE 可以显着改善空间学习和记忆能力。 SFE 可以显着调节丙二醛 (MDA)、超氧化物歧化酶 (SOD) 和晚期糖基化终末产物 (AGE)。它还改善了肝脏的病理异常。此外，SFE的抗衰老机制表明，通过代谢组学技术共发现了10个潜在的生物标志物，涉及6条代谢途径。其中，SFE能显着提高D-谷氨酰胺和D-谷氨酸的水平。此外，通过商业试剂盒和酶联免疫吸附测定法定量测定肝脏中谷氨酰胺和谷氨酸的水平，以及谷胱甘肽（GSH）合成过程中的关键氨基酸、酶和最终产物的水平。这些结果表明，谷氨酰胺-谷氨酸代谢途径的调节参与了SFE对d-gal诱导的衰老大鼠的抗衰老作用。
京公网安备 11010802027423号