Skilled reaching tasks for head-fixed mice using a robotic manipulandum.,Nature Protocols

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Skilled reaching tasks for head-fixed mice using a robotic manipulandum.
Nature Protocols ( IF 13.1 ) Pub Date : 2020-02-07 , DOI: 10.1038/s41596-019-0286-8
Mark J Wagner ₁ , Joan Savall ₁ , Tony Hyun Kim _{1,

2} , Mark J Schnitzer _{1,

3} , Liqun Luo ₁

Affiliation

Skilled forelimb behaviors are among the most important for studying motor learning in multiple species including humans. This protocol describes learned forelimb tasks for mice using a two-axis robotic manipulandum. Our device provides a highly compact adaptation of actuated planar two-axis arms that is simple and inexpensive to construct. This paradigm has been dominant for decades in primate motor neuroscience. Our device can generate arbitrary virtual movement tracks, arbitrary time-varying forces or arbitrary position- or velocity-dependent force patterns. We describe several example tasks permitted by our device, including linear movements, movement sequences and aiming movements. We provide the mechanical drawings and source code needed to assemble and control the device, and detail the procedure to train mice to use the device. Our software can be simply extended to allow users to program various customized movement assays. The device can be assembled in a few days, and the time to train mice on the tasks that we describe ranges from a few days to several weeks. Furthermore, the device is compatible with various neurophysiological techniques that require head fixation.

中文翻译：

使用机器人操作器熟练地完成头部固定小鼠的任务。

熟练的前肢行为对于研究包括人类在内的多个物种的运动学习来说是最重要的。该协议描述了使用两轴机器人操作器为小鼠学习前肢任务。我们的设备提供了高度紧凑的驱动平面两轴臂，其构造简单且成本低廉。几十年来，这种范式在灵长类运动神经科学中一直占据主导地位。我们的设备可以生成任意虚拟运动轨迹、任意随时间变化的力或任意位置或速度相关的力模式。我们描述了我们的设备允许的几个示例任务，包括线性运动、运动序列和瞄准运动。我们提供组装和控制该设备所需的机械图纸和源代码，并详细介绍了训练小鼠使用该设备的程序。我们的软件可以简单地扩展，以允许用户对各种定制的运动测定进行编程。该设备可以在几天内组装完毕，训练小鼠完成我们描述的任务的时间从几天到几周不等。此外，该设备与需要头部固定的各种神经生理学技术兼容。

更新日期：2020-02-07

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