Studies have shown that pathogenic bacteria infections induce the overproduction of reactive oxygen species (ROS) in plants. Cyanide-resistant respiration, an energy-dissipating pathway in plants, has also been induced by a pathogenic bacteria infection. However, it is unknown whether the induction of cyanide-resistant respiration under the pathogenic bacteria infection was caused by ROS. In this study, two pathogenic Erwinia strains were used to infect potato tuber, and membrane lipid peroxidation levels and the cyanide-resistant respiration capacity were determined. In addition, StAOX expression and regulation by ROS in potato tuber were analyzed. Moreover, the role of the Ca2+ pathway in regulating cyanide-resistant respiration was determined. The results showed that ROS induced cyanide-resistant respiration in potato tuber infected by Erwinia. Cyanide-resistant respiration inhibited the production of H2O2. Intracellular Ca2+ regulated the expression of calcium-dependent protein kinase (StCDPK1, StCDPK4, and StCDPK5) in potato, which indirectly controlled intracellular ROS levels. These results indicate that Ca2+ metabolism is involved in ROS-induced cyanide-resistant respiration.

中文翻译：

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活性氧在被欧文氏菌亚种感染的马铃薯中诱导抗氰化物呼吸。食肉目

研究表明，病原菌感染会导致植物中活性氧（ROS）的过度产生。抗氰呼吸是植物中的一种能量耗散途径，也是由病原菌感染诱导的。然而，尚不清楚在病原菌感染下诱导抗氰呼吸是否是由活性氧引起的。在这项研究中，两种致病性欧文氏菌被用来感染马铃薯块茎，并测定了膜脂过氧化水平和抗氰化物呼吸能力。此外，分析了马铃薯块茎中 StAOX 的表达和 ROS 的调节。此外，还确定了 Ca2+ 途径在调节抗氰呼吸作用中的作用。结果表明，ROS诱导了欧文氏菌感染的马铃薯块茎的抗氰呼吸。抗氰呼吸抑制 H2O2 的产生。细胞内Ca2+调节马铃薯钙依赖性蛋白激酶（StCDPK1、StCDPK4和StCDPK5）的表达，间接控制细胞内ROS水平。这些结果表明 Ca2+ 代谢参与了 ROS 诱导的抗氰呼吸。