2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) accumulates in human body, probably influencing adipocyte differentiation and causing various toxic effects, including wasting syndrome. Recently, orientin, a phenolic compound abundant in natural health products, has been shown to have antioxidant properties. We investigated the protective effects of orientin against TCDD-induced adipocyte dysfunction and its underlying mechanisms. In this study, orientin suppressed TCDD-induced loss of lipid accumulation. Orientin inhibited TCDD-driven decreases in the levels of peroxisome proliferator-activated receptor γ and adiponectin. Orientin also reduced TCDD-induced prostaglandin E2, and cytosolic phospholipase A2α levels, and increased TCDD-inhibited peroxisome proliferator-activated receptor gamma coactivator 1-alpha levels in 3T3-L1 adipocytes. TCDD reduced the levels of insulin receptor substrate 1 and glucose transporter 4, and decreased insulin-stimulated glucose uptake activity; however, orientin diminished these TCDD-induced effects. These results suggest that orientin may have beneficial effects on the prevention of TCDD-induced wasting syndrome and type II diabetes mellitus accompanied by insulin resistance.

中文翻译：

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Orientin降低了2,3,7,8-四氯二苯并-p-二恶英对小鼠3T3-L1脂肪细胞中脂肪形成和胰岛素信号通路的抑制作用。

2,3,7,8-四氯二苯并-对二恶英（TCDD）在人体内累积，可能影响脂肪细胞分化并引起各种毒性作用，包括消耗综合征。近来，已经证明天然植物保健品中富含的酚类化合物Orientin具有抗氧化性能。我们调查了东方蛋白对TCDD诱导的脂肪细胞功能障碍的保护作用及其潜在机制。在这项研究中，orientin抑制了TCDD诱导的脂质积累损失。Orientin抑制TCDD驱动的过氧化物酶体增殖物激活受体γ和脂联素水平的降低。Orientin还降低了3D3-L1脂肪细胞中TCDD诱导的前列腺素E2和胞质磷脂酶A2α的水平，并增加了TCDD抑制的过氧化物酶体增殖物激活的受体γ共激活物1-alpha的水平。TCDD降低了胰岛素受体底物1和葡萄糖转运蛋白4的水平，并降低了胰岛素刺激的葡萄糖摄取活性。但是，orientin减弱了这些TCDD诱导的作用。这些结果表明，orientin可能对预防TCDD诱导的消耗综合征和II型糖尿病伴胰岛素抵抗具有有益的作用。