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A first view on the unsuspected intragenus diversity of N-glycans in Chlorella microalgae.
The Plant Journal ( IF 6.2 ) Pub Date : 2020-02-07 , DOI: 10.1111/tpj.14718 Réka Mócsai 1 , Rudolf Figl 1 , Leander Sützl 2 , Silvia Fluch 3 , Friedrich Altmann 1
The Plant Journal ( IF 6.2 ) Pub Date : 2020-02-07 , DOI: 10.1111/tpj.14718 Réka Mócsai 1 , Rudolf Figl 1 , Leander Sützl 2 , Silvia Fluch 3 , Friedrich Altmann 1
Affiliation
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Chlorella microalgae are increasingly used for various purposes such as fatty acid production, wastewater processing, or as health‐promoting food supplements. A mass spectrometry‐based survey of N‐glycan structures of strain collection specimens and 80 commercial Chlorella products revealed a hitherto unseen intragenus diversity of N‐glycan structures. Differing numbers of methyl groups, pentoses, deoxyhexoses, and N ‐acetylglucosamine culminated in c. 100 different glycan masses. Thirteen clearly discernible glycan‐type groups were identified. Unexpected features included the occurrence of arabinose, of different and rare types of monosaccharide methylation (e.g. 4‐O ‐methyl‐N ‐acetylglucosamine), and substitution of the second N ‐acetylglucosamine. Analysis of barcode ITS1–5.8S–ITS2 rDNA sequences established a phylogenetic tree that essentially went hand in hand with the grouping obtained by glycan patterns. This brief prelude to microalgal N‐glycans revealed a fabulous wealth of undescribed structural features that finely differentiated Chlorella ‐like microalgae, which are notoriously poor in morphological attributes. In light of the almost identical N‐glycan structural features that exist within vertebrates or land plants, the herein discovered diversity is astonishing and argues for a selection pressure only explicable by a fundamental functional role of these glycans.
中文翻译：
关于小球藻微藻中N-聚糖的不可怀疑的内部多样性的初步观点。
小球藻微藻越来越多地用于各种目的，例如脂肪酸生产，废水处理或促进健康的食品补充剂。基于质谱的菌株收集标本和80种商业绿藻产品的N-聚糖结构调查显示，迄今为止尚未见到的N-聚糖结构的属内多样性。甲基，戊糖，脱氧己糖和N-乙酰氨基葡萄糖的数量在c中达到顶峰。100种不同的聚糖质量。确定了13个明显可识别的聚糖类型组。出乎意料的特征包括阿拉伯糖的出现，不同类型的稀有单糖甲基化（例如4- O-甲基-N-乙酰氨基葡萄糖），并替换第二个N-乙酰氨基葡萄糖。对条形码ITS1–5.8S–ITS2 rDNA序列的分析建立了系统发育树，该树基本上与通过聚糖模式获得的分组齐头并进。这是微藻类N-聚糖的简短前奏，揭示了大量难以描述的结构特征，这些结构特征可细微区分小球藻样微藻，这些藻类在形态学特性上非常差。鉴于脊椎动物或陆地植物中存在的几乎相同的N-聚糖结构特征，本文发现的多样性令人惊讶，并提出只有这些聚糖的基本功能作用才能解释的选择压力。
更新日期：2020-02-07
中文翻译：
关于小球藻微藻中N-聚糖的不可怀疑的内部多样性的初步观点。
小球藻微藻越来越多地用于各种目的，例如脂肪酸生产，废水处理或促进健康的食品补充剂。基于质谱的菌株收集标本和80种商业绿藻产品的N-聚糖结构调查显示，迄今为止尚未见到的N-聚糖结构的属内多样性。甲基，戊糖，脱氧己糖和N-乙酰氨基葡萄糖的数量在c中达到顶峰。100种不同的聚糖质量。确定了13个明显可识别的聚糖类型组。出乎意料的特征包括阿拉伯糖的出现，不同类型的稀有单糖甲基化（例如4- O-甲基-N-乙酰氨基葡萄糖），并替换第二个N-乙酰氨基葡萄糖。对条形码ITS1–5.8S–ITS2 rDNA序列的分析建立了系统发育树，该树基本上与通过聚糖模式获得的分组齐头并进。这是微藻类N-聚糖的简短前奏，揭示了大量难以描述的结构特征，这些结构特征可细微区分小球藻样微藻，这些藻类在形态学特性上非常差。鉴于脊椎动物或陆地植物中存在的几乎相同的N-聚糖结构特征，本文发现的多样性令人惊讶，并提出只有这些聚糖的基本功能作用才能解释的选择压力。
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