Cohesin Removal Reprograms Gene Expression upon Mitotic Entry.,Molecular Cell

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Cohesin Removal Reprograms Gene Expression upon Mitotic Entry.
Molecular Cell ( IF 14.5 ) Pub Date : 2020-02-07 , DOI: 10.1016/j.molcel.2020.01.023
Carlos Perea-Resa ₁ , Leah Bury ₂ , Iain M Cheeseman ₂ , Michael D Blower ₁

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As cells enter mitosis, the genome is restructured to facilitate chromosome segregation, accompanied by dramatic changes in gene expression. However, the mechanisms that underlie mitotic transcriptional regulation are unclear. In contrast to transcribed genes, centromere regions retain transcriptionally active RNA polymerase II (Pol II) in mitosis. Here, we demonstrate that chromatin-bound cohesin is necessary to retain elongating Pol II at centromeres. We find that WAPL-mediated removal of cohesin from chromosome arms during prophase is required for the dissociation of Pol II and nascent transcripts, and failure of this process dramatically alters mitotic gene expression. Removal of cohesin/Pol II from chromosome arms in prophase is important for accurate chromosome segregation and normal activation of gene expression in G1. We propose that prophase cohesin removal is a key step in reprogramming gene expression as cells transition from G2 through mitosis to G1.

中文翻译：

粘连蛋白去除会在有丝分裂进入时重新编程基因表达。

当细胞进入有丝分裂时，基因组被重组以促进染色体分离，同时伴随着基因表达的巨大变化。然而，有丝分裂转录调控的机制尚不清楚。与转录基因相反，着丝粒区域在有丝分裂中保留转录活性的 RNA 聚合酶 II (Pol II)。在这里，我们证明染色质结合的粘连蛋白对于在着丝粒处保留延长的 Pol II 是必需的。我们发现，在前期，WAPL 介导的从染色体臂上去除粘连蛋白是 Pol II 和新生转录本解离所必需的，并且该过程的失败会显着改变有丝分裂基因的表达。前期从染色体臂中去除粘连蛋白/Pol II 对于准确的染色体分离和 G1 期基因表达的正常激活非常重要。我们认为，当细胞从 G2 通过有丝分裂过渡到 G1 时，前期粘连蛋白去除是重编程基因表达的关键步骤。

更新日期：2020-02-07

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