Combinatorial Biomaterials Discovery Strategy to Identify New Macromolecular Cryoprotectants.,ACS Macro Letters

当前位置： X-MOL 学术 › ACS Macro Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Combinatorial Biomaterials Discovery Strategy to Identify New Macromolecular Cryoprotectants.
ACS Macro Letters ( IF 5.1 ) Pub Date : 2020-02-07 , DOI: 10.1021/acsmacrolett.0c00044
Christopher Stubbs ₁ , Kathryn A Murray ₁ , Toru Ishibe ₁ , Robert T Mathers ₂ , Matthew I Gibson _{1,

3}

Affiliation

Cryoprotective agents (CPAs) are typically solvents or small molecules, but there is a need for innovative CPAs to reduce toxicity and increase cell yield, for the banking and transport of cells. Here we use a photochemical high-throughput discovery platform to identify macromolecular cryoprotectants, as rational design approaches are currently limited by the lack of structure-property relationships. Using liquid handling systems, 120 unique polyampholytes were synthesized using photopolymerization with RAFT agents. Cryopreservation screening identified "hit" polymers and nonlinear trends between composition and function, highlighting the requirement for screening, with polymer aggregation being a key factor. The most active polymers reduced the volume of dimethyl sulfoxide (DMSO) required to cryopreserve a nucleated cell line, demonstrating the potential of this approach to identify materials for cell storage and transport.

中文翻译：

识别新大分子冷冻保护剂的组合生物材料发现策略。

冷冻保护剂 (CPA) 通常是溶剂或小分子，但需要创新的 CPA 来降低毒性并提高细胞产量，以用于细胞的储存和运输。在这里，我们使用光化学高通量发现平台来识别大分子冷冻保护剂，因为合理的设计方法目前因缺乏结构-特性关系而受到限制。使用液体处理系统，使用 RAFT 试剂进行光聚合合成了 120 种独特的聚两性电解质。低温保存筛选确定了“命中”聚合物和组成与功能之间的非线性趋势，突出了筛选的要求，其中聚合物聚集是一个关键因素。最活跃的聚合物减少了低温保存有核细胞系所需的二甲基亚砜 (DMSO) 的体积，

更新日期：2020-02-07

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11