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Regulation of Cohesin-Mediated Chromosome Folding by Eco1 and Other Partners.
Molecular Cell ( IF 14.5 ) Pub Date : 2020-01-28 , DOI: 10.1016/j.molcel.2020.01.019
Lise Dauban 1 , Rémi Montagne 2 , Agnès Thierry 2 , Luciana Lazar-Stefanita 3 , Nathalie Bastié 1 , Olivier Gadal 1 , Axel Cournac 2 , Romain Koszul 2 , Frédéric Beckouët 1
Affiliation  

Cohesin, a member of the SMC complex family, holds sister chromatids together but also shapes chromosomes by promoting the formation of long-range intra-chromatid loops, a process proposed to be mediated by DNA loop extrusion. Here we describe the roles of three cohesin partners, Pds5, Wpl1, and Eco1, in loop formation along either unreplicated or mitotic Saccharomyces cerevisiae chromosomes. Pds5 limits the size of DNA loops via two different pathways: the canonical Wpl1-mediated releasing activity and an Eco1-dependent mechanism. In the absence of Pds5, the main barrier to DNA loop expansion appears to be the centromere. Our data also show that Eco1 acetyl-transferase inhibits the translocase activity that powers loop formation and contributes to the positioning of loops through a mechanism that is distinguishable from its role in cohesion establishment. This study reveals that the mechanisms regulating cohesin-dependent chromatin loops are conserved among eukaryotes while promoting different functions.

中文翻译:


Eco1 和其他合作伙伴对粘连蛋白介导的染色体折叠的调控。



粘连蛋白 (Cohesin) 是 SMC 复合体家族的一员,它将姐妹染色单体固定在一起,还通过促进长范围染色单体内环的形成来塑造染色体,这一过程被认为是由 DNA 环挤出介导的。在这里,我们描述了三个粘连蛋白伴侣 Pds5、Wpl1 和 Eco1 在沿着未复制或有丝分裂的酿酒酵母染色体形成环中的作用。 Pds5 通过两种不同的途径限制 DNA 环的大小:典型的 Wpl1 介导的释放活性和 Eco1 依赖性机制。在缺乏 Pds5 的情况下,DNA 环扩张的主要障碍似乎是着丝粒。我们的数据还表明,Eco1 乙酰转移酶抑制转位酶活性,该活性为环形成提供动力,并通过与其在内聚力建立中的作用不同的机制有助于环的定位。这项研究表明,调节粘连蛋白依赖性染色质环的机制在真核生物中是保守的,同时促进不同的功能。
更新日期:2020-02-07
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