Insertion of channel of phi29 DNA packaging motor into polymer membrane for high-throughput sensing.,Nanomedicine: Nanotechnology, Biology and Medicine

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Insertion of channel of phi29 DNA packaging motor into polymer membrane for high-throughput sensing.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 4.2 ) Pub Date : 2020-02-06 , DOI: 10.1016/j.nano.2020.102170
Zhouxiang Ji ₁ , Michael Jordan ₂ , Lakmal Jayasinghe ₂ , Peixuan Guo ₃

Affiliation

The connector channel of bacteriophage phi29 DNA packaging motor has been inserted into the lipid bilayer membrane and has shown potential for the sensing of DNA, RNA, chemicals, peptides, and antibodies. Properties such as high solubility and large channel size have made phi29 channel an advantageous system for those applications; however, previously studied lipid membranes have short lifetimes, and they are frangible and unstable under voltages higher than 200 mV. Thus, the application of this lipid membrane platform for clinical applications is challenging. Here we report the insertion of the connector into the stable polymer membrane in MinION flow cell that contains 2048 wells for high-throughput sensing by the liposome-polymer fusion process. The successful insertion of phi29 connector was confirmed by a unique gating phenomenon. Peptide translocation through the inserted phi29 connector was also observed, revealing the potential of applying phi29 connector for high-throughput peptide sensing.

中文翻译：

将phi29 DNA包装电机的通道插入聚合物膜中以进行高通量检测。

噬菌体phi29 DNA包装电机的连接器通道已插入脂质双层膜中，并显示出潜在的DNA，RNA，化学物质，肽和抗体的感测能力。诸如高溶解度和大通道尺寸等特性使phi29通道成为那些应用的有利系统；然而，先前研究的脂质膜寿命短，并且在高于200 mV的电压下易碎且不稳定。因此，将该脂质膜平台用于临床应用具有挑战性。在这里，我们报告了将连接器插入MinION流通池中稳定的聚合物膜的过程，该膜包含2048个孔，用于通过脂质体-聚合物融合过程进行高通量传感。独特的门控现象证实了phi29连接器的成功插入。

更新日期：2020-02-07

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