MicroRNAs (miRNAs) participate in plant development and defence through post-transcriptional regulation of the target genes. However, few miRNAs were reported to regulate cotton plant disease resistance. Here, we characterized the cotton miR164-NAC100 module in the later induction stage response of the plant to Verticillium dahliae infection. The results of GUS fusing reporter and transcript identity showed that ghr-miR164 can directly cleave the mRNA of GhNAC100 in the post-transcriptional process. The ghr-miR164 positively regulated the cotton plant resistance to V. dahliae according to analyses of its over-expression and knockdown. In link with results, the knockdown of GhNAC100 increased the plant resistance to V. dahliae. Based on LUC reporter, expression analyses and yeast one-hybrid (Y1H) assays, GhNAC100 bound to the CGTA-box of GhPR3 promoter and repressed its expression, negatively regulating plant disease resistance. These results showed that the ghr-miR164 and GhNAC100 module fine-tunes plant defence through the post-transcriptional regulation, which documented that miRNAs play important roles in plant resistance to vascular disease.

中文翻译：

---

ghr-miR164 和 GhNAC100 调节棉花植物对大丽花轮枝菌的抗性

MicroRNAs (miRNAs) 通过靶基因的转录后调控参与植物发育和防御。然而，据报道很少有 miRNA 调节棉花植物的抗病性。在这里，我们在植物对大丽轮枝菌感染的后期诱导反应中表征了棉花 miR164-NAC100 模块。GUS 融合报告基因和转录本同一性的结果表明，ghr-miR164 在转录后过程中可以直接切割 GhNAC100 的 mRNA。根据对其过表达和敲低的分析，ghr-miR164 正向调节棉花植物对大丽花的抗性。与结果相关的是，GhNAC100 的敲低增加了植物对大丽花的抗性。基于 LUC 报告基因、表达分析和酵母单杂交 (Y1H) 分析，GhNAC100 与 GhPR3 启动子的 CGTA-box 结合并抑制其表达，负调控植物抗病性。这些结果表明，ghr-miR164 和 GhNAC100 模块通过转录后调控微调植物防御，这证明 miRNA 在植物抗血管疾病中发挥重要作用。