Galactomannan (GM) is a specific polysaccharide antigen of opportunistic mold fungi of the genus Aspergillus. The detection of GM in patients’ biological fluids is a reliable criterion for the diagnosis of invasive pulmonary aspergillosis, a severe disease. In this report, we evaluated the analytical capabilities of the fluorescence polarization immunoassay (FPIA) for detection of GM. Using FPIA, the high affinity of the monoclonal antibody 7B8 to the synthetic oligosaccharide β-d-Galf-[(1→5)-β-d-Galf]3-(1→6)-α-d-Manp related to the galactomannan fragment was shown. The binding constant in this interaction was found to be equal to (1.02±0.01) • 108 L mol-1. The detection limit determined for this oligosaccharide with the competitive FPIA method is 3 ng mL-1. The data obtained indicate the possibility of using FPIA for the diagnosis of invasive aspergillosis.

中文翻译：

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荧光偏振免疫法检测烟曲霉半乳甘露聚糖的潜力

半乳甘露聚糖 (GM) 是曲霉属机会性霉菌的特异性多糖抗原。在患者的生物体液中检测到 GM 是诊断侵袭性肺曲霉病这一严重疾病的可靠标准。在本报告中，我们评估了荧光偏振免疫分析 (FPIA) 检测转基因的分析能力。使用 FPIA，单克隆抗体 7B8 对合成寡糖 β-d-Galf-[(1→5)-β-d-Galf]3-(1→6)-α-d-Manp 的高亲和力与显示了半乳甘露聚糖片段。发现该相互作用中的结合常数等于 (1.02±0.01) • 108 L mol-1。使用竞争性 FPIA 方法确定的该寡糖的检测限为 3 ng mL-1。