This study investigated the effects of different concentrations of zeatin and thidiazuron on the accumulation of phenolics and activity of phenylalanine ammonia lyase gene in the micropropagated Satureja spicigera shoots. S. spicigera clones were generated from cultured nodal segments on Murashige and Skoog including vitamins medium supported with zeatin and thidiazuron concentrations. Shoots were subcultured three times in the same medium and harvested at the end of the third month. The expression levels of phenylalanine ammonia lyase gene were conducted using semi-quantitative reverse transcription polymerase chain reaction technique. Rosmarinic acid contents were determined using reverse phase-high performance liquid chromatography with a diode array detector. Genetic stability of regenerants was performed using random amplified polymorphic DNA markers. Essential oils from shoots were identified by gas chromatography–mass spectrophotometry analysis. It has been detected that 0.1 mg/L thidiazuron is more effective on the node formation and shoot elongation. Contrarily, 4.0 mg/L thidiazuron caused a significant increase in shoot multiplication. The highest biomass accumulation was calculated from medium including 1.0 mg/L zeatin. According to random amplified polymorphic DNA analysis, all banding profiles from micropropagated plants were monomorphic. Gas chromatography–mass spectrophotometry analysis showed that rising concentrations of zeatin and thidiazuron increased the thymol and decreased the carvacrol content. It was also found that rosmarinic acid production increased significantly at 1.0 mg/L zeatin application. However, thidiazuron applications had no positive effect on rosmarinic acid accumulation. Comparing phenylalanine ammonia lyase gene expression and rosmarinic acid accumulation in in vitro shoots, it was found that they were correlated.

中文翻译：

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在细胞分裂素处理的Satureja spicigera（C. Koch）boiss的芽器官发生过程中，精油成分，苯丙氨酸氨裂合酶基因表达和迷迭香酸含量的变化。芽

这项研究调查了不同浓度的玉米蛋白和噻二唑酮对微繁殖的Satureja spicigera幼芽中酚类物质的积累和苯丙氨酸氨裂解酶基因活性的影响。S. spicigera克隆是从Murashige和Skoog上的培养节节段生成的，其中包括添加了玉米素和噻唑隆浓度的维生素培养基。将芽在相同培养基中传代培养3次，并在第三个月末收获。采用半定量逆转录聚合酶链反应技术检测苯丙氨酸氨解酶基因的表达水平。使用具有二极管阵列检测器的反相高效液相色谱法测定迷迭香酸含量。使用随机扩增的多态性DNA标记进行再生子的遗传稳定性。芽中的精油通过气相色谱-质谱法鉴定。已发现0.1 mg / L噻唑隆对结节形成和枝条伸长更有效。相反，4。0 mg / L噻地隆引起的茎杆繁殖显着增加。从包含1.0 mg / L玉米素的培养基中计算出最高的生物量积累。根据随机扩增多态性DNA分析，来自微繁殖植物的所有条带图都是单态的。气相色谱-质谱法分析表明，玉米素和噻唑隆的浓度增加，百里酚增加，香芹酚含量降低。还发现，在1.0 mg / L玉米素用量下，迷迭香酸的产量显着增加。然而，噻地隆的应用对迷迭香酸的积累没有积极作用。比较体外芽中苯丙氨酸解氨酶基因的表达和迷迭香酸的积累，发现它们是相关的。