Pinus densiflora sieb. et zucc.(pine needle) is a traditional medicine used in several East Asian countries. However, the efficacy of pine needle has rarely been reported. In this study showed that the anti-proliferative effects and the mechanisms of hexane layer of pine needle MeOH extract (PNH) on gastric cancer cells. At first, PNH inhibited the proliferation of gastric cancer cells in a dose-dependent manner. Moreover, PNH treatment induced G1 phase cell cycle arrest through the increased p27^KIP1 expression and decreased cyclin dependent kinase (CDKs) activity. Furthermore, PNH treatment induced premature senescence without oncogenic stress, through the expression of p27^KIP1 and Skp2. Taken together, these results showed that PNH inhibited gastric cancer cell proliferation through the induction of G1-cell cycle arrest and premature senescence via induced p27^KIP1 expression, as controlled by Skp2 reduction. Also, PNH could be a candidate for anti-gastric cancer treatment and may be useful in the development of anti-gastric cancer drugs.

松树 et zucc。（松针）是东亚几个国家/地区使用的传统药物。但是，很少报道松针的功效。这项研究表明，松针甲醇提取物（PNH）的己烷层对胃癌细胞具有抗增殖作用及其机理。首先，PNH以剂量依赖的方式抑制胃癌细胞的增殖。此外，PNH治疗通过增加p27 ^KIP1表达和降低细胞周期蛋白依赖性激酶（CDKs）活性诱导G1期细胞周期停滞。此外，PNH处理通过表达p27 ^KIP1诱导早衰而无致癌性应激和Skp2。综上所述，这些结果表明，PNH通过诱导G1细胞周期停滞和诱导p27 ^KIP1表达而导致早衰，抑制了胃癌细胞的增殖，这受Skp2还原的控制。而且，PNH可能是抗胃癌治疗的候选药物，可能在抗胃癌药物的开发中有用。