Acetyl-CoA C-acetyltransferase synthase gene (AACT) cDNA, DNA and promoter were cloned from Sanghuangporus baumii. The gene ORF (1260 bp) encoded 419 amino acids. The AACT DNA includes five exons (1-84 bp, 140-513 bp, 570-1027 bp, 1090-1282 bp, 1344-1494 bp) and four introns (85-139 bp, 514-569 bp, 1028-1089 bp, 1283-1343 bp). The molecular weight of AACT protein is 43.40 kDa, it is hydrophilic with a theoretical isoelectric point of 8.96. Furthermore, The region of the transcription start site is 1997-2047 bp of AACT promoter, and it contained promoter elements (TATA Boxs, CAAT Boxs, CAAT-box, ABRE, G-Boxs, Sp1, MSA-like, LTR). AACT recombinant protein (43.40 KDa + Tag protein 22.68 KDa) was subjected in SDS-PAGE. AACT the transcription levels of in different development stages were investigated. The expression of AACT in primordia (2.4-fold) and 15 d mycelia (2.3- fold) were significantly higher than 9 d mycelia (contral). The expression level of the AACT downstream genes and triterpenoids content were determined at different developmental stages. Triterpenoid content reached its peak on day 15(7.21 mg/g).

中文翻译：

---

桑黄黄粉虫乙酰-CoA乙酰转移酶基因的分子克隆，鉴定和异源表达。

从桑黄桑por中克隆了乙酰辅酶A C-乙酰转移酶合酶基因（AACT）的cDNA，DNA和启动子。基因ORF（1260bp）编码419个氨基酸。AACT DNA包含五个外显子（1-84 bp，140-513 bp，570-1027 bp，1090-1282 bp，1344-1494 bp）和四个内含子（85-139 bp，514-569 bp，1028-1089 bp） ，1283-1343 bp）。AACT蛋白的分子量为43.40 kDa，具有亲水性，理论等电点为8.96。此外，转录起始位点的区域是AACT启动子的1997-2047bp，并且其包含启动子元件（TATA盒，CAAT盒，CAAT盒，ABRE，G盒，Sp1，MSA样，LTR）。在SDS-PAGE中对AACT重组蛋白（43.40 KDa +标签蛋白22.68 KDa）进行处理。研究了AACT在不同发育阶段的转录水平。AACT在原基中的表达（2。4倍）和15 d菌丝体（2.3倍）显着高于9 d菌丝体（对照）。在不同的发育阶段确定AACT下游基因的表达水平和三萜含量。三萜类化合物含量在第15天达到峰值（7.21 mg / g）。