Abstract In this work, the feasibility of ethanol production from waste pizza (WP) by enzymatic hydrolysis and fermentation was investigated. The effects of enzyme volumes (0.02-0.08 mL/L) and WP mass ratios (50−160 g/L) on the performance of enzymatic hydrolysis and ethanol fermentation were also examined. It was observed that the lowest enzyme volume (0.02 mL/L) could completely hydrolyze WP with mass ratio of 50 g/L. The reducing sugar (RS) increased obviously with increasing of WP mass ratio from 50 g/L to 160 g/L when the enzyme volume of 0.02 mL/L was applied. The WP hydrolysate was utilized as substrate for bio-ethanol production with the highest cumulative ethanol production of 27.58 g/L and ethanol yield of 0.292 g/g WP, respectively. To our knowledge, this is the first study to report ethanol production from WP by enzymatic hydrolysis and fermentation.

中文翻译：

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通过酶水解和发酵从废比萨中生产乙醇

摘要 在这项工作中，研究了通过酶水解和发酵从废比萨（WP）中生产乙醇的可行性。还研究了酶体积 (0.02-0.08 mL/L) 和 WP 质量比 (50-160 g/L) 对酶水解和乙醇发酵性能的影响。观察到最低酶体积 (0.02 mL/L) 可以完全水解 WP，质量比为 50 g/L。当酶量为0.02 mL/L时，随着WP质量比从50 g/L增加到160 g/L，还原糖(RS)明显增加。WP 水解物被用作生物乙醇生产的底物，最高累积乙醇产量分别为 27.58 g/L 和乙醇产量为 0.292 g/g WP。据我们所知，