BACKGROUND Ultraviolet (UV) fluorescent lamp (FL) was applied in mainstream riboflavin photochemical method (RPM) to inactivate pathogens in blood components. Low UV irradiance emitted by UV-FL resulted in more time to achieve effective inactivation. MATERIALS AND METHODS A novel light emitting diode (LED) UV illumination with adjustable irradiance was developed by us. Two strains of drug-resistant bacteria (DRB), pan-drug resistant Acinetobacter baumannii (PDRAB) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and used for evaluating the inactivation effectiveness of RPM using UV-LED or UV-FL against DRB in plasma or platelets. Three plasma factors and four platelet parameters were measured after treatments. RESULTS There was a linear relationship between UV-LED irradiance and electric current, the minimum UV irradiance was 24 mW/cm2, and the maximum was 258 mW/cm2. At the same UV dose of 15 J/cm2, inactivation effectiveness of UV-LED with 258 mW/cm2 against PDRAB in plasma or platelets were comparable to that of UV-FL with 16 mW/cm2, both above 98%. UV-FL treatment required 10-15 min, but UV-LED only required 1-2 min. However, MRSA showed a resistance to UV-LED (inactivation effectiveness was around 40%) compared with UV-FL (inactivation effectiveness was above 98%). The retention of fibrinogen, factor V, factor VII in plasma and platelet counts in platelets with UV-LED treatment were significantly higher than UV-FL at the same UV dose. CONCLUSION The treatment of RPM using UV-LED with high UV irradiance was able to dramatically shorten inactivation time against PDRAB in plasma or platelets and improve retention of blood components compared with UV-FL.

中文翻译：

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核黄素光化学方法中使用的一种新型紫外线照射，可灭活血液成分中的耐药细菌。

背景技术紫外（UV）荧光灯（FL）被应用于主流的核黄素光化学方法（RPM）中以灭活血液成分中的病原体。UV-FL发出的低UV辐射导致更长的时间来实现有效的灭活。材料与方法我们开发了一种可调节辐照度的新型发光二极管（LED）UV照明。培养了两株耐药细菌（DRB），泛药耐药鲍曼不动杆菌（PDRAB）和耐甲氧西林金黄色葡萄球菌（MRSA），并通过UV-LED或UV-FL评估RPM对DRB的灭活效果在血浆或血小板中。治疗后测量三个血浆因子和四个血小板参数。结果UV-LED的辐照度与电流呈线性关系，最小紫外线辐照度为24 mW / cm2，最大值为258 mW / cm2。在15 J / cm2的相同紫外线剂量下，血浆或血小板中258 mW / cm2的UV-LED对PDRAB的灭活效果与16 mW / cm2的UV-FL相当，均高于98％。UV-FL处理需要10-15分钟，而UV-LED仅需要1-2分钟。然而，与UV-FL（灭活效率高于98％）相比，MRSA显示出对UV-LED的抵抗力（灭活效率约为40％）。在相同的紫外线剂量下，经紫外线-LED处理的血浆中纤维蛋白原，因子V，因子VII在血浆中的保留量和血小板计数显着高于紫外线-FL。