The influence of different concentrations of iron, Fe (30–110 μM), molybdenum, Mo (8–20 μM), and ethylenediaminetetraacetic acid, EDTA (0.1–0.5 g L⁻¹) on the photofermentative hydrogen production and bacterial growth by Rhodobacter sphaeroides 158 DSM was investigated and discussed. A blend of pre-treated brewery (30%) and restaurant (70%) effluents was used successfully as sole medium (without using the standard medium) for the photofermentative hydrogen production, producing a cumulative biohydrogen of 83 mL. The results show that sole-additions of Fe at 70 μM, Mo at 14 μM, and co-addition of Fe:Mo at 70 μM:8 μM to the mixture of pre-treated brewery and restaurant effluents, could enhance the cumulative biohydrogen production to 140 mL (69% increased), 105 mL (27% increased), and 160 mL (93% increased), respectively. The results also revealed that the addition of EDTA should be optimized to avoid the chelation of the added metal ions (Fe, Mo). The biohydrogen production was further enhanced to 192 mL, which represent 131% increase compared to control, when the optimized EDTA of 0.2 g L⁻¹ was added the blended effluents at Fe:Mo concentrations of 70 μM:8 μM. Furthermore, the study shows that the addition of Fe, Mo and EDTA to the blended effluent enhances the biomass growth as well. Utilizing the wastewater for biohydrogen production as a sole medium could open new era for renewable energy production.

不同浓度的铁，铁（30–110μM），钼，钼（8–20μM）和乙二胺四乙酸，乙二胺四乙酸（EDTA）（0.1–0.5 g L ^-1）对光发酵产氢和红细菌细菌生长的影响球菌158对DSM进行了调查和讨论。经过预处理的啤酒厂（30％）和餐厅（70％）废水的混合物已成功用作光发酵制氢的唯一培养基（不使用标准培养基），产生的生物氢累积量为83 mL。结果表明，将70μM的Fe，14μM的Mo单独添加以及将70μM：8μM的Fe：Mo共添加到预处理的啤酒厂和餐厅废水混合物中，可以提高累积的生物氢产量分别达到140毫升（增加69％），105毫升（增加27％）和160毫升（增加93％）。结果还表明，应优化EDTA的添加，以避免添加的金属离子（Fe，Mo）发生螯合。当优化的EDTA为0时，生物氢的产量进一步提高到192 mL，与对照相比增加了131％。^-1溶液中加入铁的混合流出物：8μM：70μM的浓度莫。此外，研究表明，向混合废水中添加铁，钼和乙二胺四乙酸也可以促进生物量的增长。将废水用于生产生物氢作为唯一的媒介可以为可再生能源的生产打开新的时代。