Rationally designed and synthesized Schiff base sensor CMTAH containing coumarin fluorophores by the coupling of coumarin aldehyde and thiophene-2-carbohydrazide and acts as differentially selective chemosensor via colorimetric/ratiometric responses to Cu(II)/Zn(II) ions with fluorescence switchable strategy in aqueous media. Variation in absorption and emission spectra of CMTAH with different metal cations in aqueous media reveal high-affinity binding to Cu(II)/Zn(II) ions over other coexisting cations. Moreover, the spectrometric titrations and Job's plot curves substantiated the stoichiometric ratio of 1:1 for Cu(II)/Zn(II) complexes formation with low detection limits of 141 nM and 72 nM, respectively. Particularly, the responding mechanism of CMTAH toward Cu(II)/Zn(II) ions was investigated by density functional theory calculations. Notably, the molecular logic gates were established with the combination of CMTAH for the intelligentialized recognition of Cu(II)/Zn(II) ions. Additionally, the compatibility and permeability of CMTAH were attributed to Schiff base group, which exhibited remarkable responding and bioimaging in living cells and zebrafish. Notably, CMTAH could be used to image in Drosophila larvae with a clearly visible profile of internal organs and effectively capture Cu(II) and Zn(II) ions in living Drosophila larvae, which lay the foundation of sensors in the field of Drosophila biological imaging research.

通过香豆素醛和噻吩-2-碳酰肼的偶合，合理设计和合成了包含香豆素荧光团的席夫碱传感器CMTAH，并通过比色/比重响应对Cu（II）/ Zn（II）离子进行了比色/比例分析，并通过荧光转换策略用作差分选择性化学传感器。水性介质。CMTAH在水性介质中具有不同金属阳离子的吸收光谱和发射光谱的变化表明，与其他共存阳离子相比，CuMT / Zn（II）离子具有高亲和力。此外，分光光度滴定法和Job曲线图证实了Cu（II）/ Zn（II）配合物形成的化学计量比为1：1，检测限分别为141 nM和72 nM。特别是CMTAH的响应机制通过密度泛函理论计算研究了向Cu（II）/ Zn（II）离子的迁移。值得注意的是，结合CMTAH建立了分子逻辑门，用于智能识别Cu（II）/ Zn（II）离子。此外，CMTAH的相容性和渗透性归因于席夫碱基团，其在活细胞和斑马鱼中表现出显着的响应和生物成像。值得注意的是，CMTAH可用于图像在果蝇幼虫内部器官的一个清晰可见的轮廓和有效地捕获的Cu（II）和Zn（II）在活离子果蝇幼虫，奠定传感器的基础中的字段果蝇生物成像研究。