Nuclear pore complexes (NPCs) are main channels controlling nucleocytoplasmic transport and are composed of approximately 30 nucleoporins (NUPs). Emerging evidence suggests that some NUP genes have specialized functions that challenge the traditional view of NPCs as structures of uniform composition. Here, we analysed the role of six outer-ring components of NPC at normal and warm growth temperatures by examining their loss-of-function mutants in Arabidopsis thaliana. All six NUP subunits, NUP85, NUP96, NUP 133, NUP 160, SEH1 and HOS1, have a non-redundant temperature-influenced function in one or more of the processes, including rosette growth, leaf architecture and intracellular immune receptor-mediated disease resistance. At the molecular level, NUP85 and NUP133 are required for mRNA export only at warm temperature and play a larger role in the localization of transcription factor at warm temperature. In addition, NUP96 and HOS1 are essential for the expression of high temperature-responsive genes, which is correlated with their larger activity in facilitating nuclear accumulation of the transcription factor PIF4 at warm temperature. Our results show that subunits of NPC have differential roles at different temperatures, suggesting the existence of temperature-influenced NPC complexes and activities.

中文翻译：

---

核孔复合物组分在植物生长和免疫中具有温度影响的作用。

核孔复合物（NPC）是控制核质运输的主要途径，由大约30个核孔蛋白（NUP）组成。越来越多的证据表明，某些NUP基因具有专门的功能，这些功能挑战了传统的NPC统一组成结构的观点。在这里，我们通过检查拟南芥中功能丧失的突变体，分析了正常和温暖生长温度下NPC六个外环成分的作用。NUP85，NUP96，NUP 133，NUP 160，SEH1和HOS1的所有六个NUP亚基在一个或多个过程中均具有非冗余的温度影响功能，包括玫瑰花结生长，叶片结构和细胞内免疫受体介导的抗病性。在分子水平上 NUP85和NUP133仅在温暖的温度下才需要mRNA输出，并且在温暖的温度下在转录因子的定位中发挥更大的作用。此外，NUP96和HOS1对于高温响应基因的表达必不可少，这与其在温暖温度下促进转录因子PIF4的核积累的更大活性有关。我们的结果表明，NPC的亚基在不同温度下具有不同的作用，这表明存在温度影响的NPC复合物和活性。