Plasma‐activated water (PAW) is an effective disinfectant against a wide range of microorganisms, but its effect on food products is limited. This study aimed to investigate the fluid environment on the inactivation efficacy of PAW and its application on fish decontamination. Three organic media (fish gelatin, fish oil and fish homogenate) at 5%, 10% and 20%, and inorganic media of phosphate‐buffered saline (PBS) and water were inoculated with Pseudomonas fluorescens at a final concentration of 7 log₁₀ CFU per mL. The inoculated media were treated by an atmospheric cold plasma jet at 30 kV for 5 min and stored at 4 °C for 24 h, during which the inactivation effects were determined at different exposure times. The physicochemical properties of the media were measured. Furthermore, PAW generated for 15 min was applied to P. fluorescens‐inoculated fish. The results showed that PAW achieved a complete inactivation immediately after treatment, while there was almost no reduction for plasma‐activated PBS even after 24 h. Reduced inactivation efficacy was observed for the organic media except for fish oil, and the inactivation rates were in inverse correlation to the concentrations of organic material. PAW treatment obtained 0.4 log reduction on fish. This study revealed that the fluid environment had a great influence on the inactivation efficacy of PAW, and PAW can be a potential sanitiser for fish decontamination.

中文翻译：

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各种鱼类成分对血浆活化水灭活功效的影响

血浆活化水（PAW）是对多种微生物的有效消毒剂，但对食品的作用有限。本研究旨在探讨流体环境对PAW灭活功效的影响及其在鱼类去污中的应用。荧光假单胞菌接种了三种有机培养基（鱼明胶，鱼油和鱼匀浆），分别为5％，10％和20％，以及磷酸盐缓冲盐水（PBS）和水的无机培养基，最终浓度为7 log ₁₀ CFU。每毫升。接种的培养基用大气冷等离子体射流在30 kV下处理5分钟，并在4°C下保存24 h，在此过程中，在不同的暴露时间确定灭活效果。测量了介质的理化性质。此外，将产生15分钟的PAW应用于荧光假单胞菌接种鱼。结果表明，PAW在治疗后立即完全失活，而血浆激活的PBS甚至在24 h后也几乎没有减少。除鱼油外，其他有机介质的灭活效果均降低，灭活速率与有机物质的浓度呈负相关。PAW处理可使鱼类减少0.4 log。这项研究表明，流体环境对PAW的灭活效果有很大的影响，并且PAW可能是鱼类去污的潜在消毒剂。