Prominent Contribution of Hydrogen Peroxide to Intracellular Reactive Oxygen Species Generated upon Exposure to Naphthalene Secondary Organic Aerosols,Environmental Science & Technology Letters

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Prominent Contribution of Hydrogen Peroxide to Intracellular Reactive Oxygen Species Generated upon Exposure to Naphthalene Secondary Organic Aerosols
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2020-02-05 , DOI: 10.1021/acs.estlett.9b00773
Fobang Liu ₁ , Maria G. Saavedra ₂ , Julie A. Champion ₁ , Kathy K. Griendling ₃ , Nga L. Ng _{1,

2}

Affiliation

Exposure to particulate matter (PM) can induce the production of reactive oxygen and nitrogen species (ROS/RNS) in biological systems. Secondary organic aerosols (SOA) make up a substantial fraction of ambient fine PM. In this study, the contribution of hydrogen peroxide (H₂O₂) to intracellular ROS/RNS production upon exposure to naphthalene SOA was investigated using an oxidative stress indicator (carboxy-H₂DCFDA) and a H₂O₂ scavenger (catalase). With the addition of catalase, the ROS/RNS response was substantially suppressed, indicating that H₂O₂ was the main type of ROS contributing to the measured ROS/RNS. We also found that H₂O₂ in naphthalene SOA extracts (H₂O_2[SOA]) could rapidly diffuse into cells and contribute to intracellular ROS/RNS, while another contribution was from H₂O₂ produced by cells (H₂O_2[cell]). The contributions from H₂O_2[SOA] and H₂O_2[cell] were both determined. The results showed that H₂O_2[SOA] (3.16–4.20 ng/μg) was substantially higher than H₂O_2[cell] (1.03–1.27 ng/μg), assuming the production of H₂O₂ by cells was constant over time. These findings demonstrated that H₂O₂ was the main ROS accumulated in cells upon exposure to naphthalene SOA and that the diffusion of H₂O_2[SOA] into the cells could represent one of the main pathways contributing to intracellular ROS/RNS.

中文翻译：

过氧化氢对暴露于萘次级有机气溶胶后产生的细胞内活性氧的显着贡献

暴露于颗粒物（PM）可以诱导生物系统中活性氧和氮物质（ROS / RNS）的产生。次级有机气溶胶（SOA）占环境精细PM的很大一部分。在这项研究中，使用氧化应激指示剂（羧基-H ₂ DCFDA）和H ₂ O ₂清除剂（过氧化氢酶）研究了过氧化氢（H ₂ O ₂）对暴露于萘SOA后对细胞内ROS / RNS产生的影响。。添加过氧化氢酶后，ROS / RNS的反应得到了显着抑制，这表明H ₂ O ₂是导致被测ROS / RNS的主要ROS类型。我们还发现H ₂Ó ₂在萘SOA提取物（H ₂ ö _{2 [SOA]}）可以迅速地扩散到细胞中，并有助于细胞内ROS / RNS，而另一贡献是选自H ₂ ö ₂由细胞产生的（H ₂ ö _{2 [单元]}）。确定了H ₂ O _{2 [SOA]}和H ₂ O _{2 [cell]}的贡献。结果表明使得h ₂ ö _{2 [SOA]}（3.16-4.20纳克/微克）比H基本上非更高₂ ö _{2 [单元]}（1.03-1.27纳克/微克），假设生产h的₂ ö ₂随时间变化的细胞常数是恒定的。这些发现表明，H ₂ O ₂是暴露于萘SOA后在细胞中积累的主要ROS，并且H ₂ O _{2 [SOA]}向细胞内的扩散可能是导致细胞内ROS / RNS的主要途径之一。

更新日期：2020-02-06

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