The Xanthomonas oryzae pv. oryzae type IV pilus alignment subcomplex protein PilN contributes to regulation of bacterial surface-associated behaviors and T3SS system,Plant Pathology

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The Xanthomonas oryzae pv. oryzae type IV pilus alignment subcomplex protein PilN contributes to regulation of bacterial surface-associated behaviors and T3SS system
Plant Pathology ( IF 2.3 ) Pub Date : 2020-05-01 , DOI: 10.1111/ppa.13157
Yilang Li ₁ , Yichao Yan _{1,

2,

3} , Songge Deng ₁ , Cuiping Zhang _{1,

2,

3} , Fazal Haq _{1,

2,

3} , Tao Chen ₁ , Yingbin Li ₁ , Shengzhang Li _{1,

2,

3} , Rihuan Yang _{1,

2,

3} , Lifang Zou _{1,

2,

3} , Gongyou Chen _{1,

2,

3}

Affiliation

The gram‐negative plant pathogen Xanthomonas oryzae pv. oryzae (Xoo) is able to infect the host rice and effectively colonize in vascular tissues. The type IV pilus (T4P) is one of the major virulence factors playing an important role in migration of Xoo through host vascular tissues. Here, we identified PilN, a T4P alignment subcomplex protein, which is involved in regulation of swimming motility, and analysed its contribution to bacterial surface‐associated behaviours and virulence. We found that the pilN deletion mutant exhibited dramatically reduced twitching motility and scarcely detectable levels of T4P major pili PilA, as well as enhanced biofilm formation and exopolysaccharide (EPS) production. In addition, deletion of the pilN gene in Xoo resulted in impaired virulence in host rice and attenuated type III secretion system (T3SS) genes expression, which is independent of PilA assembly. Expression of the relevant pilN gene in trans was capable of restoring twitching motility and biofilm formation to the wild‐type levels in the pilN mutant but partially recovering EPS production and virulence. Moreover, the expression of trh and xrvA genes, which encode the HrpG positive regulators, was decreased in the pilN mutant. Our results suggest that PilN executes versatile functions in bacterial virulence and cell surface‐associated behaviours.

中文翻译：

米黄单胞菌 pv。oryzae IV 型菌毛排列亚复合蛋白 PilN 有助于调节细菌表面相关行为和 T3SS 系统

革兰氏阴性植物病原体米黄单胞菌 pv。oryzae (Xoo) 能够感染宿主水稻并在维管组织中有效定殖。IV 型菌毛 (T4P) 是主要的毒力因子之一，在 Xoo 通过宿主血管组织的迁移中起重要作用。在这里，我们鉴定了 PilN，一种 T4P 比对亚复合蛋白，参与游泳运动的调节，并分析了其对细菌表面相关行为和毒力的贡献。我们发现 pilN 缺失突变体表现出显着降低的抽搐运动和几乎检测不到的 T4P 主要菌毛 PilA 水平，以及增强的生物膜形成和胞外多糖 (EPS) 生产。此外，Xoo 中 pilN 基因的缺失导致宿主水稻毒力减弱和减毒 III 型分泌系统 (T3SS) 基因表达，这与 PilA 组装无关。相关 pilN 基因的反式表达能够将 pilN 突变体中的抽搐运动和生物膜形成恢复到野生型水平，但部分恢复 EPS 生产和毒力。此外，编码 HrpG 正调节因子的 trh 和 xrvA 基因的表达在 pilN 突变体中降低。我们的结果表明 PilN 在细菌毒力和细胞表面相关行为中执行多种功能。相关 pilN 基因的反式表达能够将 pilN 突变体中的抽搐运动和生物膜形成恢复到野生型水平，但部分恢复 EPS 生产和毒力。此外，编码 HrpG 正调节因子的 trh 和 xrvA 基因的表达在 pilN 突变体中降低。我们的结果表明 PilN 在细菌毒力和细胞表面相关行为中执行多种功能。相关 pilN 基因的反式表达能够将 pilN 突变体中的抽搐运动和生物膜形成恢复到野生型水平，但部分恢复 EPS 生产和毒力。此外，编码 HrpG 正调节因子的 trh 和 xrvA 基因的表达在 pilN 突变体中降低。我们的结果表明 PilN 在细菌毒力和细胞表面相关行为中执行多种功能。

更新日期：2020-05-01

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