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The Brassica napus GATA transcription factor BnA5.ZML1 is a stigma compatibility factor.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-17 , DOI: 10.1111/jipb.12916 Zhiqiang Duan 1 , Yatao Zhang 1 , Jinxing Tu 1 , Jinxiong Shen 1 , Bin Yi 1 , Tingdong Fu 1 , Cheng Dai 1 , Chaozhi Ma 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-03-17 , DOI: 10.1111/jipb.12916 Zhiqiang Duan 1 , Yatao Zhang 1 , Jinxing Tu 1 , Jinxiong Shen 1 , Bin Yi 1 , Tingdong Fu 1 , Cheng Dai 1 , Chaozhi Ma 1
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Self‐incompatibility (SI) is a genetic mechanism that rejects self‐pollen and thus prevents inbreeding in some hermaphroditic angiosperms. In the Brassicaceae, SI involves a pollen‐stigma recognition system controlled by a single locus known as the S locus, which consists of two highly polymorphic genes that encode S‐locus cysteine‐rich protein (SCR) and S‐receptor kinase (SRK). When self‐pollen lands on the stigma, the S‐haplotype‐specific interaction between SCR and SRK triggers SI. Here, we show that the GATA transcription factor BnA5.ZML1 suppresses SI responses in Brassica napus and is induced after compatible pollination. The loss‐of‐function mutant bna5.zml1 displays reduced self‐compatibility. In contrast, overexpression of BnA5.ZML1 in self‐incompatible stigmas leads to a partial breakdown of SI responses, suggesting that BnA5.ZML1 is a stigmatic compatibility factor. Furthermore, the expression levels of SRK and ARC1 are up‐regulated in bna5.zml1 mutants, and they are down‐regulated in BnA5.ZML1 overexpressing lines. SRK affects the cellular localization of BnA5.ZML1 through direct protein–protein interaction. Overall, our findings highlight the fundamental role of BnA5.ZML1 in SI responses in B. napus, establishing a direct interaction between BnA5.ZML1 and SRK in this process.
中文翻译：
甘蓝型油菜GATA转录因子BnA5.ZML1是柱头相容性因子。
自交不亲和（SI）是一种遗传机制，可拒绝自花粉，从而防止某些雌雄同体被子植物的近交。在十字花科中，SI涉及一个由一个称为S基因座的基因座控制的花粉-柱头识别系统，该系统由两个高度多态的基因组成，这些基因编码S-基因座富含半胱氨酸的蛋白（SCR）和S-受体激酶（SRK） 。当自花粉落在柱头上时，SCR和SRK之间特定于S型单体的相互作用会触发SI。在这里，我们表明，GATA转录因子BnA5.ZML1抑制甘蓝型油菜中的SI响应，并在相容的授粉后被诱导。功能丧失的突变体bna5.zml1显示出降低的自我兼容性。相反，BnA5.ZML1的过表达自相容性不佳的污名中的s导致SI反应的部分分解，表明BnA5.ZML1是一个污名性相容性因子。此外，在bna5.zml1突变体中SRK和ARC1的表达水平上调，而在BnA5.ZML1过表达株中它们的表达水平下调。SRK通过直接的蛋白质-蛋白质相互作用影响BnA5.ZML1的细胞定位。总体而言，我们的发现突出了BnA5.ZML1在甘蓝型油菜的SI反应中的基本作用，并在此过程中建立了BnA5.ZML1与SRK之间的直接相互作用。
更新日期：2020-03-17
中文翻译：
甘蓝型油菜GATA转录因子BnA5.ZML1是柱头相容性因子。
自交不亲和（SI）是一种遗传机制，可拒绝自花粉，从而防止某些雌雄同体被子植物的近交。在十字花科中，SI涉及一个由一个称为S基因座的基因座控制的花粉-柱头识别系统，该系统由两个高度多态的基因组成，这些基因编码S-基因座富含半胱氨酸的蛋白（SCR）和S-受体激酶（SRK） 。当自花粉落在柱头上时，SCR和SRK之间特定于S型单体的相互作用会触发SI。在这里，我们表明，GATA转录因子BnA5.ZML1抑制甘蓝型油菜中的SI响应，并在相容的授粉后被诱导。功能丧失的突变体bna5.zml1显示出降低的自我兼容性。相反，BnA5.ZML1的过表达自相容性不佳的污名中的s导致SI反应的部分分解，表明BnA5.ZML1是一个污名性相容性因子。此外，在bna5.zml1突变体中SRK和ARC1的表达水平上调，而在BnA5.ZML1过表达株中它们的表达水平下调。SRK通过直接的蛋白质-蛋白质相互作用影响BnA5.ZML1的细胞定位。总体而言，我们的发现突出了BnA5.ZML1在甘蓝型油菜的SI反应中的基本作用，并在此过程中建立了BnA5.ZML1与SRK之间的直接相互作用。
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