The toxic effect of dioxins and dioxin-like compounds have largely been studied using in vivo techniques; however, in vivo studies can be limited when rapid screening is required. Microtox® can be used as a rapid ecotoxicity assessment tool for dioxins in the environment, but studies on the assessment of dioxins and dioxin-like compounds using bioluminescent bacteria Allivibrio fischeri are limited. This study investigated the potential of using A. fischeri for assessing different dioxins and dioxin-like compounds, and the toxic effects of soils contaminated with dioxins and dioxin-like compounds were tested using different fractions of dioxins in soil to determine the appropriate way of assessing the toxic effects of contaminated soils. The results show that A. fischeri can potentially be used as a test species for rapidly evaluating toxic effects of dioxins and dioxin-like compounds in the environment. With the soil used in this study, the toxic effects of the water extracts (i.e., mobile fraction of dioxins) and the soil slurries (i.e., bioavailable fraction of dioxins) were similar to that of the controls. This suggests that the toxicity assessment of the organic extracts (i.e., total amount of dioxins) can be inappropriate in a managerial perspective, as the mobile or bioavailable fraction of contaminants in soils is often more of concern than the total amount of contaminants present in soils. Overall, when A. fischeri are to be used for a rapid toxicity assessment of dioxins-contaminated soils, different fractions of dioxins need to be assessed for better management of the contaminated soils.

中文翻译：

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使用阿氏弧菌评估土壤中2,3,7,8-TCDD，1,2,3,7,8-PeCDD和2,3,4,7,8-PeCDF的生态毒理作用

使用体内技术已大量研究了二恶英和二恶英类化合物的毒性作用。但是，当需要快速筛选时，体内研究可能会受到限制。Microtox®可以用作环境中二恶英的快速生态毒性评估工具，但使用生物发光细菌费氏阿里氏弧菌评估二恶英和类二恶英化合物的研究非常有限。这项研究调查了使用费氏曲霉评估不同二恶英和二恶英类化合物的潜力，并使用土壤中不同比例的二恶英来测试被二恶英和二恶英类化合物污染的土壤的毒性作用，以确定合适的评估方法污染土壤的毒性作用。结果表明A．费氏菌有可能被用作快速评估环境中二恶英和二恶英类化合物的毒性作用的试验物种。在本研究中使用的土壤中，水提取物（即二恶英的可移动部分）和泥浆（即二恶英的生物可利用部分）的毒性作用与对照相似。这表明，从管理的角度来看，有机提取物（即二恶英的总量）的毒性评估可能是不合适的，因为土壤中污染物的可移动或生物可利用部分通常比土壤中污染物的总量更受关注。总的来说，当将费氏曲霉用于对二恶英污染的土壤进行快速毒性评估时，需要评估不同比例的二恶英，以更好地管理被污染的土壤。