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How many samples do you need to assess mycorrhization in Tuber melanosporum orchards? A methodology for a reliable estimation
Agroforestry Systems ( IF 2.0 ) Pub Date : 2019-07-15 , DOI: 10.1007/s10457-019-00421-4
Leonardo Baciarelli Falini , Giorgio Marozzi , Andrea Onofri , Gian Maria Niccolò Benucci , Emidio Albertini , Domizia Donnini

The cultivation of Tuber melanosporum Vittad., the black truffle, recently expanded all over the world. For successful truffle production, an important factor to consider is the mycorrhization level of the planted mycorrhized-seedlings. To assess the mycorrhization level of a truffle orchard it is necessary to develop a sampling strategy. Root sampling is time-consuming and a delicate operation because it is easy to disturb the ectomycorrhizal communities of the truffle-plants. A standardized and reproducible methodology for sampling mycorrhized roots in truffle orchards has not yet been developed. Consequently, we sampled five T. melanosporum truffle orchards located in Central Italy to determine: (1) the relationship between the number of sampled plants and the presence of T. melanosporum and other ectomycorrhizal Fungi; (2) the minimum number of trees (MNT) needed to achieve a reliable mycorrhization estimate and the minimum sampling ratio (MSR) as the percentage of MNT over the total number of trees present in the truffle orchard. Using a general linear model, we calculated MNT for T. melanosporum and other Fungi in two sampling campaigns in each truffle orchard. The mean values of the MNT ranged among 12–17 and 17–18, respectively, for T. melanosporum and for other Fungi. According to our findings we suggest that at least a MSR of 12% should be considered to have a fair estimate of the mycorrhization level of a truffle plantation.

中文翻译:

您需要多少个样本来评估黑孢块茎果园的菌根形成?一种可靠估计的方法

黑松露Tuber melanosporum Vittad. 的栽培最近扩展到世界各地。对于成功的松露生产,需要考虑的一个重要因素是种植的菌根苗的菌根化水平。为了评估松露果园的菌根水平,有必要制定抽样策略。根取样是一项耗时且精细的操作,因为它很容易干扰松露植物的外生菌根群落。尚未开发出用于对松露果园中的菌根进行采样的标准化和可重复的方法。因此,我们对位于意大利中部的 5 个 T. melanosporum 松露果园进行了采样,以确定:(1)采样植物的数量与 T. melanosporum 和其他外生菌根真菌的存在之间的关系;(2) 实现可靠菌根化估计所需的最小树木数 (MNT) 和最小采样率 (MSR),即 MNT 占松露果园中树木总数的百分比。使用通用线性模型,我们在每个松露果园的两个采样活动中计算了黑孢霉和其他真菌的 MNT。对于黑色孢子菌和其他真菌,MNT 的平均值分别介于 12-17 和 17-18 之间。根据我们的研究结果,我们建议至少 12% 的 MSR 被认为是对松露种植园菌根化水平的公平估计。我们在每个松露果园的两个抽样活动中计算了黑孢霉和其他真菌的 MNT。对于黑色孢子菌和其他真菌,MNT 的平均值分别介于 12-17 和 17-18 之间。根据我们的研究结果,我们建议至少 12% 的 MSR 被认为是对松露种植园菌根化水平的公平估计。我们在每个松露果园的两个抽样活动中计算了黑孢霉和其他真菌的 MNT。对于黑色孢子菌和其他真菌,MNT 的平均值分别介于 12-17 和 17-18 之间。根据我们的研究结果,我们建议至少 12% 的 MSR 被认为是对松露种植园菌根化水平的公平估计。
更新日期:2019-07-15
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