Abrus precatorius possesses various therapeutic properties including anticancer potentials. This study evaluated the anti-proliferative activities of fractions of methanol root extract of A. precatorius on breast and cervical cancer cells and their immunomodulatory effect. Phytochemical screening was done by FTIR and GCMS. In vitro anti-proliferative effect was evaluated on human breast cancer (AU565) and cervical cancer (HeLa) cells and on murine fibroblast (NIH 3 T3) cells. Antioxidant activity was performed via DPPH radical scavenging assay. The immunomodulatory potential of fractions was evaluated by inhibition of phagocytes oxidative burst (ROS), Nitric oxide (NO) and proinflammatory cytokine TNF-α. A. precatorius fractions showed different chemical groups and were somewhat selective in antiproliferative activity against studied cancer cells. Ethyl acetate fraction showed the most significant antiproliferative activity with IC50 values of 18.10 μg/mL and 11.89 μg/mL against AU565 and HeLa cells respectively. Hexane fraction significantly (p < 0.05) inhibited HeLa cells (IC50 18.24 ± 0.16 μg/mL), whereas aqueous fraction showed mild inhibition (IC50 46.46 ± 0.14 μg/mL) on AU565 cell proliferation. All fractions showed no cytotoxicity against NIH-3 T3 murine fibroblast normal cells. All fractions showed potent and significant (p < 0.001) DPPH radical scavenging activity as well as suppressed phagocytic oxidative burst. Hexane (< 1 μg/mL), ethyl acetate (< 1 μg/mL), and butanol (5.74 μg/mL) fractions potently inhibited the cytokine TNF- α, hexane (< 1 μg/mL) and ethyl acetate (< 1 μg/mL) fractions also potently inhibited NO. The antiproliferative activities and suppressive effect on the phagocytic oxidative burst, NO and proinflammatory cytokine might be due to the synergistic actions of bioactive compounds especially flavonoids present in the assayed fractions and therefore, suggest chemotherapeutic use of A. precatorius in cancer treatment.

中文翻译：

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从甲醇根提取物级分的抗增殖和免疫调节活性相思子大号

益母草具有各种治疗特性，包括抗癌潜力。这项研究评估了A. precatorius甲醇根提取物的部分对乳腺癌和宫颈癌细胞的抗增殖活性及其免疫调节作用。植物化学筛选是通过FTIR和GCMS进行的。评价了对人乳腺癌（AU565）和子宫颈癌（HeLa）细胞以及鼠成纤维细胞（NIH 3 T3）细胞的体外抗增殖作用。通过DPPH自由基清除测定法进行抗氧化活性。通过抑制吞噬细胞的氧化爆发（ROS），一氧化氮（NO）和促炎性细胞因子TNF-α来评估级分的免疫调节潜力。百日咳杆菌组分显示不同的化学基团，并且对研究的癌细胞具有一定的选择性抗增殖活性。乙酸乙酯级分显示出最显着的抗增殖活性，对AU565细胞和HeLa细胞的IC50值分别为18.10μg/ mL和11.89μg/ mL。己烷组分显着（p <0.05）抑制HeLa细胞（IC50 18.24±0.16μg/ mL），而水相组分对AU565细胞的增殖表现出轻度抑制（IC50 46.46±0.14μg/ mL）。所有级分均未显示出对NIH-3 T3鼠成纤维细胞正常细胞的细胞毒性。所有馏分均显示出有效且显着的（p <0.001）DPPH自由基清除活性以及抑制的吞噬氧化爆发。己烷（<1μg/ mL），乙酸乙酯（<1μg/ mL）和丁醇（5.74μg/ mL）馏分有效抑制细胞因子TNF-α，己烷（<1μg/ mL）和乙酸乙酯（<1 μg/ mL）馏分也有效抑制NO。