Combination of bioceramics with polymers to fabricate nanofibrous scaffolds holds enormous potential for bone tissue regeneration. In this study, we aim to incorporate HAp nanoparticles in trace doping amount in PVA-chitosan nanofiber matrix to fabricate PVA-chitosan composite nanofibers with improved performance for application as a bone tissue regeneration material. The diameter of the fabricated composite nanofibrous mat is estimated as 300 ± 121 nm. Beads free nanofibers mat with uniform morphology was ascertained for all sample groups by scanning electron microscopy (SEM) and the overall composition was assessed using Fourier transform infrared spectroscopy (FTIR) and energy dispersive X-ray spectroscopy (EDX). SEM images showed a homogeneous distribution of HAp nanoparticles in the composite nanofibers matrix. Further, X-ray diffraction (XRD) was performed to determine the crystallinity of the fabricated scaffolds. Swelling behavior and hydrolytic degradation of nanofibrous mats were subsequently evaluated by immersing in PBS buffer at pH 7.4 at physiological temperature (37 °C). The biocompatibility study of nanofiber scaffolds was performed with MC3T3 cells. Significantly higher cellular viability was observed on HAp nanoparticles incorporated composite nanofibrous scaffold surface after 7 days of culture in comparison to scaffolds without HAp.

中文翻译：

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用于骨组织工程应用的生物活性纳米羟基磷灰石掺杂电纺 PVA-壳聚糖复合纳米纤维

将生物陶瓷与聚合物结合来制造纳米纤维支架具有巨大的骨组织再生潜力。在这项研究中，我们的目标是在 PVA-壳聚糖纳米纤维基质中掺入痕量掺杂的 HAp 纳米粒子，以制造具有改进性能的 PVA-壳聚糖复合纳米纤维，用作骨组织再生材料。制造的复合纳米纤维垫的直径估计为 300 ± 121 nm。通过扫描电子显微镜 (SEM) 确定所有样品组的具有均匀形态的无珠纳米纤维垫，并使用傅里叶变换红外光谱 (FTIR) 和能量色散 X 射线光谱 (EDX) 评估整体组成。SEM 图像显示复合纳米纤维基质中 HAp 纳米颗粒的均匀分布。更多，进行X射线衍射（XRD）以确定制造的支架的结晶度。随后通过在生理温度 (37 °C) 下浸入 pH 7.4 的 PBS 缓冲液中来评估纳米纤维垫的溶胀行为和水解降解。纳米纤维支架的生物相容性研究是用 MC3T3 细胞进行的。与不含 HAp 的支架相比，在培养 7 天后，在掺入复合纳米纤维支架表面的 HAp 纳米颗粒上观察到显着更高的细胞活力。纳米纤维支架的生物相容性研究是用 MC3T3 细胞进行的。与不含 HAp 的支架相比，在培养 7 天后，在掺入复合纳米纤维支架表面的 HAp 纳米颗粒上观察到显着更高的细胞活力。纳米纤维支架的生物相容性研究是用 MC3T3 细胞进行的。与不含 HAp 的支架相比，在培养 7 天后，在掺入复合纳米纤维支架表面的 HAp 纳米颗粒上观察到显着更高的细胞活力。