In recent years, there has been increasing consumer interest in carotenoids, particularly of marine sustainable origin with applications in the food, cosmeceutical, nutritional supplement and pharmaceutical industries. For instance, microalgae belonging to the genus Tetraselmis are known for their biotechnologically relevant carotenoid profile. The recently isolated marine microalgal strain Tetraselmis sp. CTP4 is a fast-growing, robust industrial strain, which has successfully been produced in 100-m³ photobioreactors. However, there are no reports on total carotenoid contents from this strain belonging to T. striata/convolutae clade. Although there are several reports on extraction methods targeting chlorophytes, extraction depends on the strength of cell coverings, solvent polarity and the nature of the targeted carotenoids. Therefore, this article evaluates different extraction methods targeting Tetraselmis sp. CTP4, a strain known to contain a mechanically resistant theca. Here, we propose a factorial experimental design to compare extraction of total carotenoids from wet and freeze-dried microalgal biomass using four different solvents (acetone, ethanol, methanol or tetrahydrofuran) in combination with two types of mechanical cell disruption (glass beads or dispersion). The extraction efficiency of the methods was assessed by pigment contents and profiles present in the extracts. Extraction of wet biomass by means of glass bead-assisted cell disruption using tetrahydrofuran yielded the highest amounts of lutein and β-carotene (622 ± 40 and 618 ± 32 µg g⁻¹ DW, respectively). Although acetone was slightly less efficient than tetrahydrofuran, it is preferable due to its lower costs and toxicity.

近年来，消费者对类胡萝卜素的兴趣日益增加，尤其是海洋可持续来源的类胡萝卜素，其在食品，药妆，营养补品和制药行业中的应用。例如，属于Tetraselmis属的微藻以其与生物技术有关的类胡萝卜素特征而闻名。最近分离的海洋微藻菌株Tetraselmis  sp。CTP4是一种快速增长的坚固工业菌株，已成功在100 m ^3的光生物反应器中生产。然而，尚无关于该菌株属于纹状体T. striata / convolutae的总类胡萝卜素含量的报道。进化枝。尽管有几种针对叶绿素的提取方法的报道，但提取取决于细胞覆盖物的强度，溶剂极性和目标类胡萝卜素的性质。因此，本文评估了针对四味子的不同提取方法 sp。CTP4，一种已知含有机械抗性茶皮的菌株。在这里，我们提出了一项析因实验设计，以比较使用四种不同溶剂（丙酮，乙醇，甲醇或四氢呋喃）与两种类型的机械细胞破裂（玻璃珠或分散液）组合从湿法和冷冻干燥的微藻生物质中提取总类胡萝卜素的方法。 。该方法的提取效率通过提取物中存在的色素含量和特性来评估。通过使用四氢呋喃的玻璃珠辅助细胞破碎来提取湿生物质，可得到最高量的叶黄素和β-胡萝卜素（622±40和618±32 µg g ^-1 DW）。尽管丙酮的效率略低于四氢呋喃，但由于其成本较低和毒性较低而优选。