Actinomycetes are known to produce various lytic enzymes such as chitinase which have different biotechnological aspects. Plackett–Burman design (PBD) was applied to evaluate significant components of medium to improve chitinase enzyme production. Response surface methodology was implemented to select those variables which are highly fitted in the model and helped in increasing enzyme production. A second experiment was performed as one of the major applications of statistically optimized and purified chitinase (method and result of purification not shown) for reducing root-knot nematode infection in roots known for causing diseases in plants. Among them, Meloidogyne sp. is one which is responsible for decreasing plant growth and fruit yield. For this, Meloidogyne sp. (1000 J2 stage) was used to infect Vigna radiata (mung) plant which was previously coated with formulations of purified chitinase and organic manure ratio—1:1 (w/w). From ANOVA results of PBD data analysis, it was confirmed that colloidal chitin, xylose, peptone and dihydrogen potassium phosphate were found to be significant components for chitinase enzyme production. The best combination of factors (gm) to accomplish the optimum response was found to be colloidal chitin: 1.0, xylose: 2.0, peptone: 0.6 and dihydrogen potassium phosphate: 0.25 for predicted response of 3.81. Seeds coated with purified chitinase (5 ml) isolated from Streptomyces rubiginosus SP24 helped in enhancing plant growth promotion and decreasing root-knot nematode infection. Statistically optimized seeds of mung coated with purified chitinase was used for suppressing plant-parasitic nematodes which cause severe harm to the production of crops in terms of plant growth and fruit yield.

中文翻译：

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统计控制的条件下，由红链霉菌SP24产生的几丁质酶的统计优化和纯化的几丁质酶控制辐射gna虫根结线虫感染的功效

已知放线菌产生具有不同生物技术方面的各种裂解酶，例如几丁质酶。Plackett–Burman设计（PBD）用于评估培养基的重要成分以改善几丁质酶的生产。实施了响应面方法，以选择那些高度适合模型并有助于增加酶产量的变量。进行第二个实验是统计学上优化和纯化的几丁质酶的主要应用之一（未显示纯化方法和结果），用于减少已知引起植物病害的根中的根结线虫感染。其中，Meloidogyne sp。是负责减少植物生长和果实产量的一种。为此，Meloidogyne sp。（1000 J2阶段）用于感染辐射紫（绿豆）植物，该植物先前已涂有纯化的几丁质酶和有机肥料比率为1：1（w / w）的制剂。从PBD数据分析的方差分析结果，证实胶体甲壳质，木糖，蛋白ept和磷酸二氢钾被认为是产生几丁质酶的重要成分。发现达到最佳响应的因素（gm）的最佳组合是胶体几丁质：1.0，木糖：2.0，蛋白ept：0.6和磷酸二氢钾：0.25，可预测到3.81的响应。分离自红链霉菌SP24的纯化的几丁质酶（5毫升）包被的种子有助于增强植物的生长并减少根结线虫的感染。