Noninvasive tool for optical online monitoring of individual biomass concentrations in a defined coculture.,Biotechnology and Bioengineering

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Noninvasive tool for optical online monitoring of individual biomass concentrations in a defined coculture.
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-01-13 , DOI: 10.1002/bit.27256
Bertram Geinitz ₁ , Lars Rehmann ₂ , Jochen Büchs ₁ , Lars Regestein _{1,

3}

Affiliation

Cocultures bear great potential in the conversion of complex substrates and process intensification, as well as, in the formation of unique components only available due to inter‐species interactions. Dynamic data of coculture composition is necessary for understanding and optimizing coculture systems. However, most standard online determined parameters measure the sum of all species in the reactor system. The kinetic behavior of the individual species remains unknown. Up to now, different offline methods are available to determine the culture composition, as well as the online measurement of fluorescence of genetically modified organisms. To avoid any genetic modification, a noninvasive online monitoring tool based on the scattered light spectrum was developed for microtiter plate cultivations. To demonstrate the potential, a coculture consisting of the bacterium Lactococcus lactis and the yeast Kluyveromyces marxianus was cultivated. Via partial least squares regression of scattered light spectra, the online determination of the individual biomass concentrations without further sampling and analyses is possible. The results were successfully validated by a Coulter counter‐analysis, taking advantage of the different cell sizes of both organisms. The findings prove the applicability of the new method to follow in detail the dynamics of a coculture.

中文翻译：

用于在定义的共培养物中在线在线监测单个生物质浓度的无创工具。

共培养在复杂的底物转化和过程强化以及仅由于物种间相互作用而可获得的独特组分的形成方面具有巨大潜力。共培养组成的动态数据对于理解和优化共培养系统是必要的。但是，大多数标准的在线确定参数可测量反应堆系统中所有物质的总和。单个物种的动力学行为仍然未知。到目前为止，可以使用不同的离线方法来确定培养物的组成，以及在线测量转基因生物的荧光。为了避免任何遗传修饰，开发了基于散射光谱的无创在线监测工具，用于微量滴定板培养。为了展示潜力，培养乳酸乳球菌和马克斯克鲁维酵母。通过散射光谱的偏最小二乘回归，无需进一步采样和分析就可以在线确定各个生物量的浓度。利用两种生物的不同细胞大小，通过Coulter反分析成功验证了结果。这些发现证明了该新方法适用于详细研究共培养的动力学。

更新日期：2020-03-09

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