In this report we evaluated a diagnostic algorithm, proposed by the Belgian Superior Health Council, to detect acute and past Epstein–Barr virus (EBV) infections by means of serology in donors of human body material for transplantation. The available EBV serology parameters were tested on eighty serum samples on three random access analysers: Architect i2000 SR, Liasion XL and BioPlex 2200. The EBV sero-status was determined according to the proposed algorithm and results were compared between the different analysers. Seventy one % of the samples gave concordant interpretations on the three analysers. Most of the discordant results were attributable to early antigen (EA) IgG. The knowledge of the EA IgG and heterophile antibodies (HA) IgM status provided only limited added value and was only useful to distinguish between a very early acute infection and false positivity of viral capsid antigen IgM. The diagnostic algorithm proposed by the Belgian Superior Health Council is merely directive and each individual lab remains responsible for the interpretation and implementation of test combinations for the detection of EBV infections. Our study shows the limited added value of testing for EA IgG and HA IgM, based both on clinical and technical performance.

中文翻译：

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人体供体的EBV血清学解释：是否需要进行早期抗原IgG和嗜异性抗体测试？

在本报告中，我们评估了比利时超级健康委员会提出的诊断算法，该算法通过血清学检测供体供体中的急性和过去的爱泼斯坦-巴尔病毒（EBV）感染。在三个随机访问分析仪（包括Architect i2000 SR，Liasion XL和BioPlex 2200）上对80个血清样本进行了可用的EBV血清学参数测试。根据拟议的算法确定EBV血清状态，并在不同分析仪之间进行比较。71％的样本对三位分析员给出了一致的解释。大多数不一致的结果可归因于早期抗原（EA）IgG。EA IgG和嗜异性抗体（HA）IgM状态的知识仅提供有限的附加值，并且仅用于区分病毒衣壳抗原IgM的非常早期的急性感染和假阳性。比利时高级卫生委员会提出的诊断算法仅是指令，每个实验室都负责解释和实施用于检测EBV感染的测试组合。我们的研究表明，基于临床和技术性能，测试EA IgG和HA IgM的附加价值有限。比利时高级卫生委员会提出的诊断算法仅是指令，每个实验室都负责解释和实施用于检测EBV感染的测试组合。我们的研究表明，基于临床和技术性能，测试EA IgG和HA IgM的附加价值有限。比利时高级卫生委员会提出的诊断算法仅是指令，每个实验室都负责解释和实施用于检测EBV感染的测试组合。我们的研究表明，基于临床和技术性能，测试EA IgG和HA IgM的附加价值有限。