Cold plasma is a new agricultural technology demonstrating wide potential application in plants. Previous studies have elucidated that the cold plasma pre-treatment of seeds improves seed germination and early growth. However, the effects of cold plasma treatment on vegetative propagation, including rooting, remains unclear. In this study, effects of cold plasma treatment on rooting percentage, physiochemical properties in stolon cuttings and physiological characteristics in the rooting process of centipedegrass (Eremochloa ophiuroides (Munro.) Hack.) stolon cuttings were investigated. The centipedegrass stolon cuttings were pre-treated with 0, 150, 300 and 450 W of cold plasma for 15 s. Cold plasma treatments positively stimulated rooting of stolon cuttings, and the 300 W treatment yielded the best effect. Rooting percentage, numbers of adventitious roots, total root length, total root surface area, total root volume, average root diameter, and dry weights of roots were significantly improved by cold plasma treatments. The conductivity and water uptake increased by cold plasma treatments; the auxin (IAA) content and activities of polyphenol oxidase and peroxidase activities improved, and the IAA-oxidase activity decreased; the soluble sugar and protein contents increased after cold plasma treatment. The results of this study not only showed that cold plasma pre-treatment may improve rooting of vegetations but also provided inspiration and support for the application of cold plasma in vegetative propagation of plant.

中文翻译：

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蜈蚣 (Eremochloa ophiuroides (Munro.) Hack.) 冷等离子体对匍匐茎插条预处理的生根及相关生理特征响应

冷等离子体是一种新的农业技术，在植物中具有广泛的应用潜力。先前的研究已经阐明，种子的冷等离子体预处理可以改善种子发芽和早期生长。然而，冷等离子体处理对包括生根在内的营养繁殖的影响仍不清楚。本研究研究了冷等离子体处理对蜈蚣(Eremochloa ophiuroides (Munro.) Hack.)匍匐茎扦插生根率、理化特性和生根过程生理特性的影响。蜈蚣匍匐茎段分别用 0、150、300 和 450 W 的冷等离子体预处理 15 秒。冷等离子体处理积极刺激匍匐茎插条生根，300 W 处理效果最好。生根百分比，冷等离子体处理显着改善了不定根数、总根长、总根表面积、总根体积、平均根直径和根干重。冷等离子体处理增加了电导率和吸水率；生长素（IAA）含量及多酚氧化酶活性和过氧化物酶活性提高，IAA-氧化酶活性降低；冷等离子体处理后可溶性糖和蛋白质含量增加。本研究结果不仅表明冷等离子体预处理可以促进植物生根，而且为冷等离子体在植物营养繁殖中的应用提供了启示和支持。冷等离子体处理显着改善了根的干重。冷等离子体处理增加了电导率和吸水率；生长素（IAA）含量及多酚氧化酶活性和过氧化物酶活性提高，IAA-氧化酶活性降低；冷等离子体处理后可溶性糖和蛋白质含量增加。本研究结果不仅表明冷等离子体预处理可以促进植物生根，而且为冷等离子体在植物营养繁殖中的应用提供了启示和支持。冷等离子体处理显着改善了根的干重。冷等离子体处理增加了电导率和吸水率；生长素（IAA）含量及多酚氧化酶活性和过氧化物酶活性提高，IAA-氧化酶活性降低；冷等离子体处理后可溶性糖和蛋白质含量增加。本研究结果不仅表明冷等离子体预处理可以促进植物生根，而且为冷等离子体在植物营养繁殖中的应用提供了启示和支持。冷等离子体处理后可溶性糖和蛋白质含量增加。本研究结果不仅表明冷等离子体预处理可以促进植物生根，而且为冷等离子体在植物营养繁殖中的应用提供了启示和支持。冷等离子体处理后可溶性糖和蛋白质含量增加。本研究结果不仅表明冷等离子体预处理可以促进植物生根，而且为冷等离子体在植物营养繁殖中的应用提供了启示和支持。