For bacteria sampling studies, various collection methods have been used to identify bacteria. To obtain accurate information about bacteria, high quality samples should be obtained. In order to obtain a high quality sample, a stable and large number of DNA copies must be collected. This study compared the efficiency of different methods of bacterial gDNA extraction and bacteria collection according to swabbing solution volumes and types. The efficiency of bacterial genomic DNA extraction was compared using a AccuPrep^® Genomic DNA Extraction kit, a QIAamp^® DNA Mini kit, and a MOBIO^® DNeasy PowerSoil kit. The DNA Mini kit was shown to extract the highest amount of gDNA, and sub-experiments were conducted using this kit. Phosphate-buffered saline and phosphate-buffered saline with 0.1% Tween 20 were used as collection solutions of various volumes (0, 40, 50, 60, 70, 80, 90, 100, 110, and 120 μL) using cotton swabs. Bacteria collection efficiency was highest when 70 μL PBS was used. The target strains collected in this experiment were Staphylococcus aureus and Escherichia coli, and these were quantified using the number of colony-forming units, DNA concentrations, and the number of DNA copies. These results can be used to efficiently bacterial collection for experiments in various fields.

中文翻译：

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擦拭溶液体积和 gDNA 提取试剂盒对刚性表面 DNA 回收的比较


对于细菌取样研究，已使用各种收集方法来鉴定细菌。为了获得有关细菌的准确信息，应获得高质量的样本。为了获得高质量的样本，必须收集稳定且大量的DNA拷贝。本研究根据拭子溶液体积和类型比较了不同细菌 gDNA 提取和细菌收集方法的效率。使用 AccuPrep ^®基因组 DNA 提取试剂盒、QIAamp ^® DNA Mini 试剂盒和 MOBIO ^® DNeasy PowerSoil 试剂盒比较了细菌基因组 DNA 提取的效率。 DNA Mini 试剂盒显示可提取最高量的 gDNA，并使用该试剂盒进行了子实验。使用棉签使用磷酸盐缓冲盐水和含 0.1% Tween 20 的磷酸盐缓冲盐水作为不同体积（0、40、50、60、70、80、90、100、110 和 120 μL）的收集溶液。使用 70 μL PBS 时细菌收集效率最高。本实验中收集的目标菌株是金黄色葡萄球菌和大肠杆菌，并使用菌落形成单位数、DNA 浓度和 DNA 拷贝数对这些菌株进行定量。这些结果可用于有效收集细菌以用于各个领域的实验。