For bacteria sampling studies, various collection methods have been used to identify bacteria. To obtain accurate information about bacteria, high quality samples should be obtained. In order to obtain a high quality sample, a stable and large number of DNA copies must be collected. This study compared the efficiency of different methods of bacterial gDNA extraction and bacteria collection according to swabbing solution volumes and types. The efficiency of bacterial genomic DNA extraction was compared using a AccuPrep^® Genomic DNA Extraction kit, a QIAamp^® DNA Mini kit, and a MOBIO^® DNeasy PowerSoil kit. The DNA Mini kit was shown to extract the highest amount of gDNA, and sub-experiments were conducted using this kit. Phosphate-buffered saline and phosphate-buffered saline with 0.1% Tween 20 were used as collection solutions of various volumes (0, 40, 50, 60, 70, 80, 90, 100, 110, and 120 μL) using cotton swabs. Bacteria collection efficiency was highest when 70 μL PBS was used. The target strains collected in this experiment were Staphylococcus aureus and Escherichia coli, and these were quantified using the number of colony-forming units, DNA concentrations, and the number of DNA copies. These results can be used to efficiently bacterial collection for experiments in various fields.

中文翻译：

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擦拭溶液体积和gDNA提取试剂盒从刚性表面回收DNA的比较

对于细菌采样研究，已使用各种收集方法来识别细菌。为了获得有关细菌的准确信息，应获取高质量的样品。为了获得高质量的样品，必须收集稳定且大量的DNA拷贝。这项研究根据擦拭溶液的体积和类型比较了不同方法提取细菌gDNA和收集细菌的效率。细菌基因组DNA提取的效率是使用AccuPrep相比^®基因组DNA提取试剂盒，一个的QIAamp ^® DNA Mini试剂盒，和一个MOBIO ^®DNeasy PowerSoil套件。DNA Mini试剂盒显示出提取的gDNA最高，并且使用该试剂盒进行了子实验。使用棉签将磷酸盐缓冲盐水和含0.1％Tween 20的磷酸盐缓冲盐水用作各种体积（0、40、50、60、70、80、90、100、110和120μL）的收集溶液。当使用70μLPBS时，细菌收集效率最高。在该实验中收集的目标菌株是金黄色葡萄球菌和大肠杆菌，并使用菌落形成单位数，DNA浓度和DNA拷贝数对它们进行定量。这些结果可用于有效地收集细菌，用于各个领域的实验。