This study aimed to develop a determination method of mycophenolic acid (MPA) in biological samples using salting-out liquid–liquid microextraction from human plasma samples based on the formation of a fluorescent complex with terbium ion and its application in quantification of the MPA in clinical samples. MPA extracted from plasma samples by salting-out liquid–liquid microextraction after protein precipitation by acetonitrile. Then, the fluorescence intensity was measured at 545 nm using an excitation wavelength of 347 nm after optimization of extraction parameters. Under the optimum conditions for the developed method, it provided a linear range between 0.5 and 5.0 µg/mL with a correlation coefficient (R²) of 0.998, and then, the validation was performed according to the FDA guideline and the developed method could quantify MPA concentration in real plasma samples. The results showed that linearity, accuracy, and precision of the developed analysis method were suitable for the determination of the MPA in plasma samples.

中文翻译：

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盐析液-液微萃取测定血浆样品中的麦考酚酸

这项研究的目的是基于盐和liquid离子荧光复合物的形成及其在临床中对MPA定量的应用，开发一种从人血浆样品中盐析液液微萃取法测定生物样品中麦考酚酸（MPA）的方法。样品。乙腈沉淀蛋白质后，通过盐析液-液微萃取从血浆样品中提取MPA。然后，在优化提取参数后，使用347 nm的激发波长在545 nm处测量荧光强度。在所开发方法的最佳条件下，其线性范围为0.5至5.0 µg / mL，相关系数为（R ²）（0.998），然后根据FDA指南进行验证，开发的方法可以定量实际血浆样品中的MPA浓度。结果表明，所建立的分析方法的线性，准确度和精密度均适用于血浆样品中MPA的测定。