Heparan sulfate (HS) with various sulfation patterns is one of important modulators of cancer cell fate through interacting with numerous growth factors. Here we found HS 2-O sulfotransferase 1 (HS2ST1) was downregulated at both mRNA and protein levels during granulocytic differentiation of SKM-1 leukemia cells and also HS (glucosamine) 3-O sulfotransferase 3A (HS3ST3A) in epithelial-mesenchymal transition (EMT) of A549 lung cancer cells, meanwhile, cell-surface HS recognized by anti-HS antibody was also changed in both cancer cell lines. Further, HS3ST3A was negatively correlated with the in vitro cell metastasis capability of A549 cells confirmed by RNA interference technology, wound-healing assay and in vitro Matrigel invasion assay. Together, specific HS sulfotransferases may serve as molecular markers and targets for cancer treatment.

中文翻译：

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硫酸乙酰肝素硫酸盐转移酶和细胞表面硫酸乙酰肝素在SKM-1细胞粒细胞分化和A549细胞上皮-间质转化过程中的变化

通过与多种生长因子相互作用，具有多种硫酸化模式的硫酸乙酰肝素（HS）是癌细胞命运的重要调节剂之一。在这里，我们发现在SKM-1白血病细胞的粒细胞分化过程中，HS 2-O磺基转移酶1（HS2ST1）在mRNA和蛋白质水平上均下调，在上皮-间质转化（EMT）中还存在HS（葡萄糖胺）3-O磺基转移酶3A（HS3ST3A）。同时，两种癌细胞系中被抗-HS抗体识别的细胞表面HS也发生了变化。此外，通过RNA干扰技术，伤口愈合试验和体外证实，HS3ST3A与A549细胞的体外细胞转移能力呈负相关。基质胶侵袭测定。在一起，特定的HS磺基转移酶可以充当癌症治疗的分子标记物和靶标。