Conservation Genetics Resources ( IF 0.9 ) Pub Date : 2019-10-19 , DOI: 10.1007/s12686-019-01115-w Celine Blanc-Jolivet , Malte Mader , Henri-Noël Bouda , Erwan Guichoux , Germain Yene , Emmanuel Opuni-Frimpong , Bernd Degen
The vulnerable African timber species Nauclea diderrichii is threatened because of its overexploitation and illegal trade. We sampled individuals across Ghana, Nigeria, Cameroon, Congo Brazzaville, Gabon and Democratic Republic of Congo and performed restriction associated DNA sequencing and low coverage MiSeq genome sequencing to discover SNP and INDEL loci. After MassARRAY genotyping of 95 individuals covering the sampled distribution range at 205 loci, a set of 88 loci were selected according to their differentiation among geographical regions. It is the first time that molecular markers are described for N. diderrichii and it should encourage genetic studies in this valuable species.
中文翻译:
非洲木材物种Nauclea diderrichii SNP标记的开发
脆弱的非洲木材物种Nauclea diderrichii由于其过度开发和非法贸易而受到威胁。我们对加纳,尼日利亚,喀麦隆,刚果布拉柴维尔,加蓬和刚果民主共和国的个体进行了采样,并进行了限制性相关DNA测序和低覆盖率MiSeq基因组测序,以发现SNP和INDEL位点。在对95个个体进行MassARRAY基因分型后,这些个体覆盖了205个基因座的抽样分布范围,根据他们在地理区域之间的差异选择了88个基因座。这是首次描述杜氏猪笼草的分子标记,它应该鼓励对该有价值物种的遗传学研究。