Objective

Lately, lncRNAs have been proposed to function in the radio-sensitivity of tumor cells, yet the role of lncRNA GAS5 in that of esophageal squamous cell carcinoma (ESCC) has scarcely been studied. This study aims to examine GAS5's effects on ESCC cell radio-sensitivity.

Methods

GAS5, miR-21 and RECK expression in radiation-sensitive and radiation-resistant ESCC tissues, and TE-1 and TE-1-R cells was determined. TE-1 and TE-1-R cells were treated with pcDNA-GAS5 or miR-21 inhibitors to figure out their roles in ESCC cell proliferation, radio-sensitivity, and apoptosis via gain- and loss-of-function experiments.

Results

We found underexpressed GAS5 and RECK, and overexpressed miR-21 in ESCC. GAS5 elevation and miR-21 inhibition reduced viability and the colony formation ability, and enhanced the apoptosis of ESCC cells under radiation.

Conclusion

Our study reveals that GAS5 elevation up-regulates RECK expression by down-regulating miR-21 to increase ESCC cell apoptosis after radiation therapy, thus enhancing cell radio-sensitivity.

中文翻译：

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较长的非编码RNA GAS5的升高和microRNA-21的敲低可上调RECK表达，以增强放疗后食管鳞状细胞癌细胞的放射敏感性。

目的

近年来，已提出lncRNA在肿瘤细胞的放射敏感性中起作用，然而，几乎未研究lncRNA GAS5在食管鳞状细胞癌（ESCC）中的作用。这项研究旨在检查GAS5对ESCC细胞放射敏感性的影响。

方法

确定了辐射敏感和抗辐射的ESCC组织以及TE-1和TE-1-R细胞中GAS5，miR-21和RECK的表达。用pcDNA-GAS5或miR-21抑制剂对TE-1和TE-1-R细胞进行处理，以通过功能获得和丧失实验确定它们在ESCC细胞增殖，放射敏感性和细胞凋亡中的作用。

结果

我们在ESCC中发现GAS5和RECK表达不足，而miR-21表达过度。GAS5升高和miR-21抑制降低了活力和集落形成能力，并增强了辐射下ESCC细胞的凋亡。

结论

我们的研究表明，GAS5升高通过下调miR-21来增加RECC的表达，从而增加放射治疗后ESCC细胞的凋亡，从而增强细胞的放射敏感性。