Bacillus cereus NJSZ-13, an endophytic bacterium with nematicidal activity, was isolated from stems of healthy Pinus elliottii Engelm. Colonization of P. massoniana Lamb. by endophytic B. cereus was studied using scanning electron microscopy and confocal laser scanning microscopy. After the plasmid pGFP78 containing the green fluorescent protein (GFP) gene was transformed into the NJSZ-13 strain, the NJSZ-13:gfp showed the same nematicidal activity and growth curve as the wild-type strain, and the plasmid pGFP78 was stably maintained in strain NJSZ-13 for at least 96 h of bacterial cultivation on medium without antibiotics. After inoculation into Masson pine roots, colonization of the NJSZ-13:gfp strain in plant roots and stems was visualized using confocal laser scanning and the strain was enumerated in inoculated roots and stems. These results suggest that NJSZ-13:gfp is an efficient colonizer of Masson pine and can transfer vertically from roots to stems.

蜡状芽孢杆菌NJSZ-1​​3，一种具有杀线虫活性的内生细菌，是从健康的松树松树的茎中分离出来的。马尾松羔羊的定殖。用扫描电镜和共聚焦激光扫描显微镜研究了蜡状芽孢杆菌的内生芽孢杆菌。将包含绿色荧光蛋白（GFP）基因的质粒pGFP78转化为NJSZ-1​​3菌株后，NJSZ-1​​3：gfp的杀线虫活性和生长曲线与野生型菌株相同，并且质粒pGFP78得以稳定维持在不含抗生素的培养基上，在NJSZ-1​​3菌株中进行至少96 h的细菌培养。接种到马尾松根中后，NJSZ-1​​3：gfp定殖使用共聚焦激光扫描观察植物根和茎中的菌株，并在接种的根和茎中列举该菌株。这些结果表明，NJSZ-1​​3：gfp是马尾松的有效定居者，可以从根部垂直转移到茎部。