Abstract

Barbaram (1), a neolactam together with other five known compunds (2–6) was isolated from the EtOAc-soluble fraction of an EtOH extract of the root and stem of Lycium barbarum by using silica gel, Sephadex LH-20 column chromatography and semi-preparative RP-C₁₈ HPLC. Based on HR-TOF-MS, NMR spectral data, quantum chemistry ECD calculations and referencing to the data reported earlier, the structures of these compounds (1–6) were determined, specially including the absolute configuration of the neolactam (1). Compounds 2 and 4 showed significant anti-inflammatory activity in LPS-induced RAW 264.7 macrophages with the IC₅₀ values of 8.98 ± 0.57 μM, 6.68 ± 0.77 μM.

摘要

Barbaram（1），与其他五个已知侃，陈万平（一个neolactam一起2 - 6）从根的乙醇提取物的乙酸乙酯可溶级分中分离和干的枸杞通过使用硅胶，交联葡聚糖LH-20柱色谱和半制备型RP-C ₁₈ HPLC。根据HR-TOF-MS，NMR光谱数据，量子化学计算ECD和引用到数据之前的报道，这些化合物（的结构1 - 6）进行了测定，特别是包括neolactam（的绝对构型1）。化合物2和4在LPS诱导的RAW 264.7巨噬细胞中显示出显着的抗炎活性，IC ₅₀值分别为8.98±0.57μM，6.68±0.77μM。